ATP1A1/ATP1A2/ATP1A3 Antibody (H-3): sc-48345

Na+/K+-ATPase α Antibody (H-3) is a mouse monoclonal IgG2b kappa light chain antibody engineered to recognize Na+/K+-ATPase α subunit, also known as ATP1A1, across mouse, rat, human, and canine species. Na+/K+-ATPase α monoclonal antibody (H-3) performs effectively in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Available in non-conjugated form and conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, Na+/K+-ATPase α (H-3) antibody suits diverse research needs. Na+/K+-ATPase, commonly referred to as the sodium-potassium pump, is a crucial plasma membrane protein responsible for maintaining cellular homeostasis by actively transporting three sodium ions out of the cell and two potassium ions into the cell against their electrochemical gradients. This activity is essential for regulating cellular volume, maintaining the resting membrane potential, and facilitating secondary active transport of numerous solutes. Located in the plasma membrane, Na+/K+-ATPase plays a vital role in key physiological processes including nerve impulse transmission, muscle contraction, and kidney function. The protein complex comprises multiple isoforms of α, β, and γ subunits, where α subunit contains binding sites for ATP and transported ions, β subunit ensures proper folding and membrane localization, and γ subunit modulates enzyme′s ATP affinity in specific tissues. Understanding Na+/K+-ATPase α1 localization and function is fundamental for investigating various cellular mechanisms and disease states such as hypertension, heart disease, and neurological disorders. Na+/K+-ATPase α (H-3) monoclonal antibody enables researchers to effectively study expression, regulation, and role of this vital protein in different biological contexts, advancing insights into cellular physiology and pathology.

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ATP1A1/ATP1A2/ATP1A3 Antibody (H-3) References:

1. Functional role and immunocytochemical localization of the gamma a and gamma b forms of the Na,K-ATPase gamma subunit.  |  Pu, HX., et al. 2001. J Biol Chem. 276: 20370-8. PMID: 11278761
2. Chemical modification as an approach to elucidation of sodium pump structure-function relations.  |  Pedemonte, CH. and Kaplan, JH. 1990. Am J Physiol. 258: C1-23. PMID: 2154108
3. The sodium pump needs its beta subunit.  |  McDonough, AA., et al. 1990. FASEB J. 4: 1598-605. PMID: 2156741
4. Mutual dependence of Na,K-ATPase alpha- and beta-subunits for correct posttranslational processing and intracellular transport.  |  Ackermann, U. and Geering, K. 1990. FEBS Lett. 269: 105-8. PMID: 2167238
5. A proteolipid associated with Na,K-ATPase is not essential for ATPase activity.  |  Hardwicke, PM. and Freytag, JW. 1981. Biochem Biophys Res Commun. 102: 250-7. PMID: 6272791
6. Functional significance of the beta-subunit for heterodimeric P-type ATPases.  |  Chow, DC. and Forte, JG. 1995. J Exp Biol. 198: 1-17. PMID: 7891030
7. Na+,K(+)-ATPase.  |  Lingrel, JB. and Kuntzweiler, T. 1994. J Biol Chem. 269: 19659-62. PMID: 8051040
8. Subunit requirements for expression of functional sodium pumps in yeast cells.  |  Scheiner-Bobis, G. and Farley, RA. 1994. Biochim Biophys Acta. 1193: 226-34. PMID: 8054343
9. The alpha-subunit of the Na,K-ATPase has catalytic activity independent of the beta-subunit.  |  Blanco, G., et al. 1994. J Biol Chem. 269: 23420-5. PMID: 8089106
10. Expression, targeting, and assembly of functional Na,K-ATPase polypeptides in baculovirus-infected insect cells.  |  DeTomaso, AW., et al. 1993. J Biol Chem. 268: 1470-8. PMID: 8380413
11. Molecular cloning and immunological characterization of the gamma polypeptide, a small protein associated with the Na,K-ATPase.  |  Mercer, RW., et al. 1993. J Cell Biol. 121: 579-86. PMID: 8387529
12. Structure and function of the Na,K pump: ten years of molecular biology.  |  Pressley, TA. 1996. Miner Electrolyte Metab. 22: 264-71. PMID: 8933497

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ATP1A1/ATP1A2/ATP1A3 Antibody (H-3) has 92 citations in a variety of scientific publications.

Primary antibodies like Anti-ATP1A1/ATP1A2/ATP1A3 Antibody (H-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ATP1A1/ATP1A2/ATP1A3 Antibody (H-3) Specifications

Alternate Product Name: ATP1A1

Product Clone Name: H-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 551-850

Epitope Target: Na+/K+-ATPase α1

Epitope Species: human

Product Reactivity/Detection: Na⁺/K⁺-ATPase α1, 2 and 3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 92 citations in various scientific publications

Available Conjugates/Forms for ATP1A1/ATP1A2/ATP1A3 Antibody (H-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin