N-SMase2 Antibody (G-6): sc-166637

N-SMase2 Antibody (G-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects N-SMase2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). N-SMase2 (G-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. N-SMase2, also known as NSMASE2 or sphingomyelin phosphodiesterase 3 (SMPD3), is a 655 amino acid enzyme that plays a crucial role in lipid metabolism by catalyzing the hydrolysis of sphingomyelin into ceramide and phosphocholine. This reaction is vital for regulating cell growth and apoptosis, making N-SMase2 an important player in cellular signaling pathways. N-SMase2 is primarily localized to the membrane of the Golgi apparatus, where activation occurs by unsaturated fatty acids and phosphatidylserine, linking N-SMase2 to processes such as growth suppression and postnatal development. Notably, N-SMase2 expression increases during the G0/G1 phases of the cell cycle, with optimal enzymatic activity at a slightly basic pH of 7.5. Deficiencies in N-SMase2 have been associated with chondrodysplasia, a genetic disorder that results in impaired bone growth, leading to short stature and underdeveloped joints, highlighting N-SMase2′s significance in skeletal development and overall health.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

N-SMase2 Antibody (G-6) References:

1. Cloning and characterization of the mammalian brain-specific, Mg2+-dependent neutral sphingomyelinase.  |  Hofmann, K., et al. 2000. Proc Natl Acad Sci U S A. 97: 5895-900. PMID: 10823942
2. Biochemical properties of mammalian neutral sphingomyelinase 2 and its role in sphingolipid metabolism.  |  Marchesini, N., et al. 2003. J Biol Chem. 278: 13775-83. PMID: 12566438
3. Neutral sphingomyelinase 2 (smpd3) in the control of postnatal growth and development.  |  Stoffel, W., et al. 2005. Proc Natl Acad Sci U S A. 102: 4554-9. PMID: 15764706
4. A deletion in the gene encoding sphingomyelin phosphodiesterase 3 (Smpd3) results in osteogenesis and dentinogenesis imperfecta in the mouse.  |  Aubin, I., et al. 2005. Nat Genet. 37: 803-5. PMID: 16025116
5. Endothelial nitric oxide synthase activation by tumor necrosis factor alpha through neutral sphingomyelinase 2, sphingosine kinase 1, and sphingosine 1 phosphate receptors: a novel pathway relevant to the pathophysiology of endothelium.  |  De Palma, C., et al. 2006. Arterioscler Thromb Vasc Biol. 26: 99-105. PMID: 16269668
6. Novel tumor necrosis factor-responsive mammalian neutral sphingomyelinase-3 is a C-tail-anchored protein.  |  Krut, O., et al. 2006. J Biol Chem. 281: 13784-13793. PMID: 16517606
7. nSMase2 activation and trafficking are modulated by oxidative stress to induce apoptosis.  |  Levy, M., et al. 2006. Biochem Biophys Res Commun. 344: 900-5. PMID: 16631623
8. Neutral sphingomyelinases and nSMase2: bridging the gaps.  |  Clarke, CJ. and Hannun, YA. 2006. Biochim Biophys Acta. 1758: 1893-901. PMID: 16938269
9. Neutral sphingomyelinase (SMPD3) deficiency causes a novel form of chondrodysplasia and dwarfism that is rescued by Col2A1-driven smpd3 transgene expression.  |  Stoffel, W., et al. 2007. Am J Pathol. 171: 153-61. PMID: 17591962
10. Off-target function of the Sonic hedgehog inhibitor cyclopamine in mediating apoptosis via nitric oxide-dependent neutral sphingomyelinase 2/ceramide induction.  |  Meyers-Needham, M., et al. 2012. Mol Cancer Ther. 11: 1092-102. PMID: 22452947
11. Identification of Heat Shock Protein 60 as a Regulator of Neutral Sphingomyelinase 2 and Its Role in Dopamine Uptake.  |  Ahn, KH., et al. 2013. PLoS One. 8: e67216. PMID: 23840630
12. Neonatal stroke enhances interaction of microglia-derived extracellular vesicles with microglial cells.  |  Lecuyer, M., et al. 2021. Neurobiol Dis. 157: 105431. PMID: 34153465

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-N-SMase2 Antibody (G-6) has 33 citations in a variety of scientific publications.

Primary antibodies like Anti-N-SMase2 Antibody (G-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

N-SMase2 Antibody (G-6) Specifications

Alternate Product Name: SMPD3

Product Clone Name: G-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 461-655

Epitope Target: N-SMase2

Epitope Species: human

Product Reactivity/Detection: sphingomyelin phosphodiesterase 3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 33 citations in various scientific publications

Available Conjugates/Forms for N-SMase2 Antibody (G-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin