N-Shc Antibody (H-7): sc-365598

N-Shc Antibody (H-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects N-Shc protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-N-Shc antibody (H-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. N-Shc protein plays a crucial role in neuronal signaling, as N-Shc is specifically expressed in the brain and contains two phosphotyrosine-binding domains and a single Src homology 2 (SH2) domain. This unique structure allows N-Shc to interact with activated growth factor receptors, facilitating the transmission of signals that are vital for neuronal development and function. By coupling activated receptors to downstream signaling pathways, N-Shc contributes to the regulation of various cellular processes, including cell proliferation and differentiation. N-Shc′s ability to mediate these signaling events underscores N-Shc′s importance in neurobiology and potential implications in understanding neurodegenerative diseases and brain tumors.

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N-Shc Antibody (H-7) References:

1. Comparative expression profiles of Trk receptors and Shc-related phosphotyrosine adapters during retinal development: potential roles of N-Shc/ShcC in brain-derived neurotrophic factor signal transduction and modulation.  |  Nakazawa, T., et al. 2002. J Neurosci Res. 68: 668-80. PMID: 12111828
2. Shc proteins are phosphorylated and regulated by the v-Src and v-Fps protein-tyrosine kinases.  |  McGlade, J., et al. 1992. Proc Natl Acad Sci U S A. 89: 8869-73. PMID: 1409579
3. Hippocampal synaptic modulation by the phosphotyrosine adapter protein ShcC/N-Shc via interaction with the NMDA receptor.  |  Miyamoto, Y., et al. 2005. J Neurosci. 25: 1826-35. PMID: 15716419
4. RAI(ShcC/N-Shc)-dependent recruitment of GAB 1 to RET oncoproteins potentiates PI 3-K signalling in thyroid tumors.  |  De Falco, V., et al. 2005. Oncogene. 24: 6303-13. PMID: 15940252
5. A novel transforming protein (SHC) with an SH2 domain is implicated in mitogenic signal transduction.  |  Pelicci, G., et al. 1992. Cell. 70: 93-104. PMID: 1623525
6. Platelet-derived growth factor (PDGF)-dependent association of phospholipase C-gamma with the PDGF receptor signaling complex.  |  Morrison, DK., et al. 1990. Mol Cell Biol. 10: 2359-66. PMID: 1691440
7. SH2 and SH3 domains: elements that control interactions of cytoplasmic signaling proteins.  |  Koch, CA., et al. 1991. Science. 252: 668-74. PMID: 1708916
8. Oncogenes and signal transduction.  |  Cantley, LC., et al. 1991. Cell. 64: 281-302. PMID: 1846320
9. Signal transduction by receptors with tyrosine kinase activity.  |  Ullrich, A. and Schlessinger, J. 1990. Cell. 61: 203-12. PMID: 2158859
10. Phosphorylation of RasGRP1 by Shc3 prevents RasGRP1 degradation and contributes to Ras/c-Jun activation in hepatocellular carcinoma.  |  Zhang, X., et al. 2024. Mol Cell Biochem. 479: 2307-2321. PMID: 37646951
11. Interaction of Shc with the zeta chain of the T cell receptor upon T cell activation.  |  Ravichandran, KS., et al. 1993. Science. 262: 902-5. PMID: 8235613
12. N-Shc: a neural-specific adapter molecule that mediates signaling from neurotrophin/Trk to Ras/MAPK pathway.  |  Nakamura, T., et al. 1996. Oncogene. 13: 1111-21. PMID: 8808684

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-N-Shc Antibody (H-7) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-N-Shc Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

N-Shc Antibody (H-7) Specifications

Alternate Product Name: N-Shc

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 191-330

Epitope Target: N-Shc

Epitope Species: human

Product Reactivity/Detection: N-Shc p52 and p64

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for N-Shc Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin