N-RAP Antibody (H-5): sc-514480
- N-RAP Antibody (H-5) is a mouse monoclonal IgG1 κ N-RAP antibody provided at 200 µg/ml
- raised against amino acids 169-468 mapping within an internal region of N-RAP of human origin
- N-RAP Antibody (H-5) is recommended for detection of N-RAP of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-N-RAP Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for N-RAP Antibody (H-5) for WB applications. These reagents are now offered in bundles with N-RAP Antibody (H-5) (see ordering information below).
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N-RAP Antibody (H-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects N-RAP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). N-RAP (H-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. N-RAP, or nebulin-related anchoring protein, is a crucial component in muscle tissue, consisting of 1,730 amino acids and playing a significant role in myofibril assembly and force transmission in cardiomyocytes. N-RAP is specifically localized to the intercalated disks of cardiac muscle and the myotendinous junctions of skeletal muscle, where N-RAP is exclusively expressed. N-RAP structure includes one N-terminal LIM domain and forty-four nebulin repeats, which are essential for N-RAP function in maintaining the integrity and organization of the sarcomere. N-RAP interacts with several key proteins during myofibril assembly, including α-actinin, Filamin 2, Actin, KBTBD10, Talin-1, and vinculin, highlighting N-RAP importance in muscle development and function. The gene encoding N-RAP is located on human chromosome 10q25.3 and mouse chromosome 19 D2, underscoring N-RAP evolutionary conservation across species.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
N-RAP Antibody (H-5) References:
- Molecular interactions of N-RAP, a nebulin-related protein of striated muscle myotendon junctions and intercalated disks. | Luo, G., et al. 1999. Biochemistry. 38: 6135-43. PMID: 10320340
- Ultrastructural and biochemical localization of N-RAP at the interface between myofibrils and intercalated disks in the mouse heart. | Zhang, JQ., et al. 2001. Biochemistry. 40: 14898-906. PMID: 11732910
- New N-RAP-binding partners alpha-actinin, filamin and Krp1 detected by yeast two-hybrid screening: implications for myofibril assembly. | Lu, S., et al. 2003. J Cell Sci. 116: 2169-78. PMID: 12692149
- Genomic organization, alternative splicing, and expression of human and mouse N-RAP, a nebulin-related LIM protein of striated muscle. | Mohiddin, SA., et al. 2003. Cell Motil Cytoskeleton. 55: 200-12. PMID: 12789664
- Nebulin: the nebulous, multifunctional giant of striated muscle. | McElhinny, AS., et al. 2003. Trends Cardiovasc Med. 13: 195-201. PMID: 12837582
- N-RAP expression during mouse heart development. | Lu, S., et al. 2005. Dev Dyn. 233: 201-12. PMID: 15765519
- Targeted disruption of N-RAP gene function by RNA interference: a role for N-RAP in myofibril organization. | Dhume, A., et al. 2006. Cell Motil Cytoskeleton. 63: 493-511. PMID: 16767749
- Role of nonmuscle myosin IIB and N-RAP in cell spreading and myofibril assembly in primary mouse cardiomyocytes. | Lu, S. and Horowits, R. 2008. Cell Motil Cytoskeleton. 65: 747-61. PMID: 18615632
- Expression and alternative splicing of N-RAP during mouse skeletal muscle development. | Lu, S., et al. 2008. Cell Motil Cytoskeleton. 65: 945-54. PMID: 18792955
- Myofibril assembly visualized by imaging N-RAP, alpha-actinin, and actin in living cardiomyocytes. | Manisastry, SM., et al. 2009. Exp Cell Res. 315: 2126-39. PMID: 19233165
- Complete cDNA sequence and tissue localization of N-RAP, a novel nebulin-related protein of striated muscle. | Luo, G., et al. 1997. Cell Motil Cytoskeleton. 38: 75-90. PMID: 9295142
- Mapping of the gene (NRAP) encoding N-RAP in the mouse and human genomes. | Luo, G., et al. 1997. Genomics. 45: 229-32. PMID: 9339382
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
N-RAP Antibody (H-5) | sc-514480 | 200 µg/ml | $322.00 | |||
N-RAP Antibody (H-5): m-IgG Fc BP-HRP Bundle | sc-527907 | 200 µg Ab; 10 µg BP | $361.00 | |||
N-RAP Antibody (H-5): m-IgG1 BP-HRP Bundle | sc-533280 | 200 µg Ab; 20 µg BP | $361.00 | |||
N-RAP Antibody (H-5) AC | sc-514480 AC | 500 µg/ml, 25% agarose | $424.00 | |||
N-RAP Antibody (H-5) HRP | sc-514480 HRP | 200 µg/ml | $322.00 | |||
N-RAP Antibody (H-5) FITC | sc-514480 FITC | 200 µg/ml | $336.00 | |||
N-RAP Antibody (H-5) PE | sc-514480 PE | 200 µg/ml | $349.00 | |||
N-RAP Antibody (H-5) Alexa Fluor® 488 | sc-514480 AF488 | 200 µg/ml | $364.00 | |||
N-RAP Antibody (H-5) Alexa Fluor® 546 | sc-514480 AF546 | 200 µg/ml | $364.00 | |||
N-RAP Antibody (H-5) Alexa Fluor® 594 | sc-514480 AF594 | 200 µg/ml | $364.00 | |||
N-RAP Antibody (H-5) Alexa Fluor® 647 | sc-514480 AF647 | 200 µg/ml | $364.00 | |||
N-RAP Antibody (H-5) Alexa Fluor® 680 | sc-514480 AF680 | 200 µg/ml | $364.00 | |||
N-RAP Antibody (H-5) Alexa Fluor® 790 | sc-514480 AF790 | 200 µg/ml | $364.00 |