N/R-cadherin Antibody (H-4): sc-271386

N/R-cadherin Antibody (H-4) is a mouse monoclonal IgG2b kappa light chain antibody designed to detect both N-cadherin and R-cadherin proteins across multiple species, including mouse, rat, human, avian, equine, canine, and bovine, utilizing applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). N/R-cadherin monoclonal antibody (H-4) targets a precise epitope within amino acids 808-837 of the C-terminal cytoplasmic domain of human N-cadherin, ensuring accurate and reliable binding. N/R-cadherin proteins are crucial members of the cadherin family, which are calcium-dependent adhesion molecules essential for mediating cell-cell adhesion, maintaining tissue structure, and facilitating morphogenesis. Located primarily at the plasma membrane, N/R-cadherin plays a pivotal role in the formation and maintenance of adherens junctions, which are vital for cellular communication and the structural integrity of tissues. The structure of N/R-cadherin comprises multiple extracellular cadherin repeats that mediate homophilic binding between cells, a single transmembrane domain that anchors the protein to the cell membrane, and a relatively short cytoplasmic domain that interacts with various intracellular proteins. These structural features are critical for the protein′s function in cell adhesion and signaling. Post-translational modifications of N/R-cadherin, such as phosphorylation, are important as they regulate protein interactions and stability, influencing processes like cell migration, differentiation, and tissue remodeling. N/R-cadherin interacts with several key proteins, including beta-catenin, which links cadherins to the actin cytoskeleton, α-catenin, which connects beta-catenin to actin filaments, and p120 catenin, which regulates cadherin stability and turnover at the cell membrane. These interactions are essential for the dynamic regulation of cell adhesion and the maintenance of cellular architecture. Anti-N/R-cadherin antibody (H-4) is available in both non-conjugated form and a variety of conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. N/R-cadherin (H-4) antibody is instrumental in research areas such as developmental biology, cancer metastasis, and neurobiology, where understanding the precise mechanisms of cell adhesion and signaling is fundamental.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-N/R-cadherin Antibody (H-4) has 41 citations in a variety of scientific publications.

Primary antibodies like Anti-N/R-cadherin Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

N/R-cadherin Antibody (H-4) Specifications

Alternate Product Name: CDH2

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 808-837

Epitope Target: N-cadherin

Epitope Species: human

Product Reactivity/Detection: N-cadherin and R-cadherin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and avian

Additional Non-Mammalian Reactivity: avian

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 41 citations in various scientific publications

Available Conjugates/Forms for N/R-cadherin Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred N-cadherin antibody is N-cadherin Antibody (13A9): sc-59987.