N/R-cadherin Antibody (H-4) recommended for detection of N-cadherin and R-cadherin of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA

N/R-cadherin Antibody (H-4): sc-271386

N/R-cadherin Antibody (H-4) is rated 4.1 out of 5 by 28.
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Datasheets

    • N/R-cadherin Antibody (H-4) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 808-837 within a C-terminal cytoplasmic domain of N-cadherin of human origin
    • N/R-cadherin Antibody (H-4) is recommended for detection of N-cadherin and R-cadherin of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and avian
    • Anti-N/R-cadherin Antibody (H-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-271386 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of N/R-cadherin (H-4): sc-271386.
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Can the peptide sc-271386 P be used in Western Blot as a positive control?

Asked by: AbPolly
Thank you for your question. The blocking peptide sc-271386 P is a short synthetic peptide intended for use as a negative control by preadsorption. It is not appropriate for use as a positive control in Western Blot.
Answered by: Technical Service
Date published: 2017-02-02
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Rated 1 out of 5 by from Didnot work for WB did not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 5 out of 5 by from good This is antibody is specific great antibody for Western blotting.
Date published: 2017-09-07
Rated 5 out of 5 by from Excellent antibody The bond is single, clear, and the background is low. Compared with other antibody, it is stable and effective.
Date published: 2017-09-06
Rated 3 out of 5 by from single band but not very shap I used this antibody in the ratio 1:1000 in pancreatic cancer cell line and I got a single band but it was not very shap.
Date published: 2017-08-30
Rated 4 out of 5 by from Good antibody I used this antibody in a dilution 1:1000, in most of the case, a clear band can be acquired.
Date published: 2017-08-29
Rated 3 out of 5 by from Function in general I tried the product and found the result was not strong by western.
Date published: 2017-08-24
Rated 1 out of 5 by from trashiness dilution ratio1:300 in WB for lovo,sw480,sw48,hct116 and other CRC. 2 days of incubation can get nothing!!!
Date published: 2017-08-22
Rated 5 out of 5 by from nice antibody Using this antibody is Western Blot, the result looks good.
Date published: 2017-07-11
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