MYLK Antibody (MLJ-18): sc-69870

MYLK Antibody (MLJ-18) is a mouse monoclonal IgG1 antibody that detects myosin light chain kinase (MYLK) in human samples through western blotting (WB). MYLK is a crucial enzyme that plays a significant role in muscle contraction by phosphorylating myosin regulatory light chains, which facilitates the interaction between myosin and actin filaments, ultimately leading to contractile activity. This process is vital not only in skeletal and cardiac muscles but also in smooth muscle tissues, where MYLK isoforms contribute to various physiological functions, including vascular tone regulation and gastrointestinal motility. The MYLK gene encodes multiple isoforms, including those specific to smooth muscle and non-muscle tissues, as well as telokin, a smaller isoform that stabilizes unphosphorylated myosin filaments, thereby influencing muscle contraction dynamics. MYLK is also a target for p21-activated kinases (PAKs), with PAK1 interaction leading to a decrease in MYLK activity and subsequent myosin light chain phosphorylation, highlighting the regulatory mechanisms that control muscle contraction and relaxation. The versatility of MYLK expression across different tissues underscores its importance in both normal physiology and potential pathophysiological conditions, making anti-MYLK antibody (MLJ-18) an essential tool for researchers studying muscle biology and related disorders.

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MYLK Antibody (MLJ-18) References:

1. An intronic MYLK variant associated with inflammatory lung disease regulates promoter activity of the smooth muscle myosin light chain kinase isoform.  |  Han, YJ., et al. 2012. J Mol Med (Berl). 90: 299-308. PMID: 22015949
2. MYLK and MYL9 expression in non-small cell lung cancer identified by bioinformatics analysis of public expression data.  |  Tan, X. and Chen, M. 2014. Tumour Biol. 35: 12189-200. PMID: 25179839
3. The ZEB1/miR-200c feedback loop regulates invasion via actin interacting proteins MYLK and TKS5.  |  Sundararajan, V., et al. 2015. Oncotarget. 6: 27083-96. PMID: 26334100
4. ROCK2 and MYLK variants under hypobaric hypoxic environment of high altitude associate with high altitude pulmonary edema and adaptation.  |  Pandey, P., et al. 2015. Appl Clin Genet. 8: 257-67. PMID: 26586960
5. Two novel MYLK nonsense mutations causing thoracic aortic aneurysms/dissections in patients without apparent family history.  |  Luyckx, I., et al. 2017. Clin Genet. 92: 444-446. PMID: 28401540
6. Loss-of-Function Variants in MYLK Cause Recessive Megacystis Microcolon Intestinal Hypoperistalsis Syndrome.  |  Halim, D., et al. 2017. Am J Hum Genet. 101: 123-129. PMID: 28602422
7. Myosin light chain kinase (MYLK) coding polymorphisms modulate human lung endothelial cell barrier responses via altered tyrosine phosphorylation, spatial localization, and lamellipodial protrusions.  |  Wang, T., et al. 2018. Pulm Circ. 8: 2045894018764171. PMID: 29480069
8. GPR65 inhibits human trophoblast cell adhesion through upregulation of MYLK and downregulation of fibronectin via cAMP-ERK signaling in a low pH environment.  |  Mao, J., et al. 2023. Cell Commun Signal. 21: 238. PMID: 37723567
9. Activation of the ROCK/MYLK Pathway Affects Complex Molecular and Morphological Changes of the Trabecular Meshwork Associated With Ocular Hypertension.  |  Hsu, CC., et al. 2024. Invest Ophthalmol Vis Sci. 65: 17. PMID: 39115865
10. A De Novo Missense MYLK Variant Leading to Nonsyndromic Thoracic Aortic Aneurysm and Dissection Identified by Segregation Analysis.  |  Nishijo, D., et al. 2024. Case Rep Genet. 2024: 4281972. PMID: 39185084

The preferred MYLK antibody is MYLK Antibody (A-8): sc-365352.