MYLK Antibody (A-8): sc-365352

MYLK Antibody (A-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects MYLK protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). MYLK Antibody (A-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MYLK protein plays a crucial role in muscle contraction by phosphorylating myosin regulatory light chains, which facilitates interaction between myosin and actin filaments, leading to contractile activity. This function is particularly important in smooth muscle tissues, where MYLK isoforms contribute to regulation of vascular tone and gastrointestinal motility. MYLK gene encodes several isoforms, including those specific to smooth muscle and non-muscle tissues, as well as telokin, a small isoform that stabilizes unphosphorylated myosin filaments. Multiple transcript variants for MYLK gene highlight diverse regulatory roles across different tissues. MYLK interacts with p21-activated kinases (PAKs), with PAK1 binding leading to a decrease in MYLK activity and subsequent myosin light chain phosphorylation, further emphasizing MYLK′s significance in cellular signaling pathways.

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MYLK Antibody (A-8) References:

1. Inhibition of myosin light chain kinase by p21-activated kinase.  |  Sanders, LC., et al. 1999. Science. 283: 2083-5. PMID: 10092231
2. A single human myosin light chain kinase gene (MLCK; MYLK).  |  Lazar, V. and Garcia, JG. 1999. Genomics. 57: 256-67. PMID: 10198165
3. Analysis of the kinase-related protein gene found at human chromosome 3q21 in a multi-gene cluster: organization, expression, alternative splicing, and polymorphic marker.  |  Watterson, DM., et al. 1999. J Cell Biochem. 75: 481-91. PMID: 10536370
4. Site-specific phosphorylation and point mutations of telokin modulate its Ca2+-desensitizing effect in smooth muscle.  |  Walker, LA., et al. 2001. J Biol Chem. 276: 24519-24. PMID: 11346659
5. Isolation of the cDNA encoding rat skeletal muscle myosin light chain kinase. Sequence and tissue distribution.  |  Roush, CL., et al. 1988. J Biol Chem. 263: 10510-6. PMID: 2839493
6. Silencing of long noncoding RNA SRRM2-AS exerts suppressive effects on angiogenesis in nasopharyngeal carcinoma via activating MYLK-mediated cGMP-PKG signaling pathway.  |  Chen, S., et al. 2020. J Cell Physiol. 235: 7757-7768. PMID: 31742692
7. Circular RNA MYLK promotes tumour growth and metastasis via modulating miR-513a-5p/VEGFC signalling in renal cell carcinoma.  |  Li, J., et al. 2020. J Cell Mol Med. 24: 6609-6621. PMID: 32342645
8. Circular RNA MYLK Promotes Hepatocellular Carcinoma Progression Through the miR29a/KMT5C Signaling Pathway.  |  Gao, J., et al. 2020. Onco Targets Ther. 13: 8615-8627. PMID: 32904604
9. MYLK*FLNB and DOCK1*LAMA2 gene-gene interactions associated with rheumatoid arthritis in the focal adhesion pathway.  |  Veyssiere, M., et al. 2024. Front Genet. 15: 1375036. PMID: 38803542
10. Human calcium-calmodulin dependent protein kinase I: cDNA cloning, domain structure and activation by phosphorylation at threonine-177 by calcium-calmodulin dependent protein kinase I kinase.  |  Haribabu, B., et al. 1995. EMBO J. 14: 3679-86. PMID: 7641687
11. The human myosin light chain kinase (MLCK) from hippocampus: cloning, sequencing, expression, and localization to 3qcen-q21.  |  Potier, MC., et al. 1995. Genomics. 29: 562-70. PMID: 8575746
12. Myosin light chain kinase in endothelium: molecular cloning and regulation.  |  Garcia, JG., et al. 1997. Am J Respir Cell Mol Biol. 16: 489-94. PMID: 9160829

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MYLK Antibody (A-8) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-MYLK Antibody (A-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MYLK Antibody (A-8) Specifications

Alternate Product Name: MYLK

Product Clone Name: A-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 21-47

Epitope Target: MYLK

Epitope Species: human

Product Reactivity/Detection: most MYLK isoforms

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for MYLK Antibody (A-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin