Myf-5 Antibody (B-2): sc-518039

Myf-5 Antibody (B-2) is a mouse monoclonal IgG3 kappa light chain antibody that detects Myf-5 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Myf-5 antibody (B-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Myf-5, a member of the basic helix-loop-helix (bHLH) transcription factor family, plays a crucial role in muscle development by regulating myogenic cell differentiation. Myf-5 (B-2) monoclonal antibody recognizes a protein essential for precursor cell commitment to myogenic lineage, with expression tightly controlled during muscle cell differentiation. Myf-5 forms heterodimers with other bHLH proteins, such as Myo D, enhancing function in binding to E-box sequences in regulatory regions of muscle-specific genes, thereby facilitating transcriptional activation necessary for muscle formation. Understanding Myf-5′s role in muscle development provides insights into muscle regeneration and potential therapeutic targets for muscle-wasting diseases.

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Myf-5 Antibody (B-2) References:

1. Herculin, a fourth member of the MyoD family of myogenic regulatory genes.  |  Miner, JH. and Wold, B. 1990. Proc Natl Acad Sci U S A. 87: 1089-93. PMID: 2300571
2. Myf-6, a new member of the human gene family of myogenic determination factors: evidence for a gene cluster on chromosome 12.  |  Braun, T., et al. 1990. EMBO J. 9: 821-31. PMID: 2311584
3. Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD.  |  Wright, WE., et al. 1989. Cell. 56: 607-17. PMID: 2537150
4. Identification of MRF4: a new member of the muscle regulatory factor gene family.  |  Rhodes, SJ. and Konieczny, SF. 1989. Genes Dev. 3: 2050-61. PMID: 2560751
5. A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts.  |  Braun, T., et al. 1989. EMBO J. 8: 701-9. PMID: 2721498
6. Function of the myogenic regulatory factors Myf5, MyoD, Myogenin and MRF4 in skeletal muscle, satellite cells and regenerative myogenesis.  |  Zammit, PS. 2017. Semin Cell Dev Biol. 72: 19-32. PMID: 29127046
7. HLH forced dimers: tethering MyoD to E47 generates a dominant positive myogenic factor insulated from negative regulation by Id.  |  Neuhold, LA. and Wold, B. 1993. Cell. 74: 1033-42. PMID: 7691411
8. Inactivation of Myf-6 and Myf-5 genes in mice leads to alterations in skeletal muscle development.  |  Braun, T. and Arnold, HH. 1995. EMBO J. 14: 1176-86. PMID: 7720708
9. MyoD or Myf-5 is required for the formation of skeletal muscle.  |  Rudnicki, MA., et al. 1993. Cell. 75: 1351-9. PMID: 8269513
10. A cellular factor stimulates the DNA-binding activity of MyoD and E47.  |  Thayer, MJ. and Weintraub, H. 1993. Proc Natl Acad Sci U S A. 90: 6483-7. PMID: 8393567
11. Use of a conditional MyoD transcription factor in studies of MyoD trans-activation and muscle determination.  |  Hollenberg, SM., et al. 1993. Proc Natl Acad Sci U S A. 90: 8028-32. PMID: 8396258
12. Different MRF4 knockout alleles differentially disrupt Myf-5 expression: cis-regulatory interactions at the MRF4/Myf-5 locus.  |  Yoon, JK., et al. 1997. Dev Biol. 188: 349-62. PMID: 9268580

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Myf-5 Antibody (B-2) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Myf-5 Antibody (B-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Myf-5 Antibody (B-2) Specifications

Alternate Product Name: MYF5

Product Clone Name: B-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 19-38

Epitope Target: Myf-5

Epitope Species: human

Product Reactivity/Detection: Myf-5

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Myf-5 Antibody (B-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Myf-5 antibody is Myf-5 Antibody (B-10): sc-518056.