Date published: 2025-9-5

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MyD88 抗体 (E-11): sc-74532

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说明书
  • MyD88 抗体 E-11 是小鼠单克隆 IgG2b κ,MyD88抗体, 在123篇文献中引用,规格为200 µg/ml
  • 免疫human物种的MyD88的氨基酸1-296
  • MyD88 抗体 (E-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 MyD88
  • 抗MyD88抗体(E-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MyD88 (E-11): sc-74532免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRPm-IgGκ BP-HRP 是 MyD88 抗体 (E-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MyD88 抗体 (E-11) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

MyD88抗体(E-11)是一种IgG2b κ小鼠单克隆MyD88抗体(也称为先天免疫信号转导适配体抗体),可通过蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学(石蜡)和酶联免疫吸附试验检测小鼠、大鼠和人类来源的MyD88蛋白。MyD88抗体(E-11)既有非偶联形式的抗MyD88抗体,也有多种偶联形式的抗MyD88抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。白细胞介素-1(IL-1)诱导的NFκB通路激活是通过IL-1受体和随后IL-1受体相关激酶(IRAK)的磷酸化介导的。髓样分化蛋白MyD88最初被鉴定为在IL-6诱导的生长停滞和终末分化后,在髓样白血病细胞中上调的蛋白。现在已知MyD88作为IRAK与IL-1受体结合的适配蛋白发挥作用。MyD88在功能上与果蝇Toll信号通路中的适配蛋白tube同源,这两种蛋白都是Toll/IL-1R超家族的成员。MyD88含有一个特征性的N端死亡结构域,该结构域对NFκB的激活至关重要,并且与一个相邻的Toll/IL-1R同源结构域(TIR结构域)相邻。这些结构域共同使MyD88能够与IRAK和IL-1受体复合物发生蛋白质-蛋白质相互作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MyD88 抗体 (E-11) 参考文献:

  1. 人类淋巴瘤中具有致癌活性的MYD88突变。  |  Ngo, VN., et al. 2011. Nature. 470: 115-9. PMID: 21179087
  2. 华氏巨球蛋白血症中的MYD88 L265P体细胞突变。  |  Treon, SP., et al. 2012. N Engl J Med. 367: 826-33. PMID: 22931316
  3. IRAK4支架整合了TLR4驱动的TRIF和MYD88信号通路。  |  Pereira, M., et al. 2022. Cell Rep. 40: 111225. PMID: 35977521
  4. 五味子乙素B通过靶向MyD88并抑制MyD88依赖性炎症来减轻糖尿病性心肌病。  |  Luo, W., et al. 2022. Adv Sci (Weinh). 9: e2202590. PMID: 36180407
  5. 在细菌性骨感染中,骨细胞通过 MYD88 信号直接调节骨溶解。  |  Yoshimoto, T., et al. 2022. Nat Commun. 13: 6648. PMID: 36333322
  6. 果蝇胚胎中背腹信号的传导需要细胞激酶与膜相关蛋白管的相互作用。  |  Galindo, RL., et al. 1995. Development. 121: 2209-18. PMID: 7635064
  7. 人类 MyD88 的分子特征和模块分析。  |  Hardiman, G., et al. 1996. Oncogene. 13: 2467-75. PMID: 8957090
  8. IRAK(Pelle)家族成员IRAK-2和MyD88是IL-1信号转导的近端介质。  |  Muzio, M., et al. 1997. Science. 278: 1612-5. PMID: 9374458
  9. MyD88:一种将 IRAK 募集到 IL-1 受体复合物的适配器。  |  Wesche, H., et al. 1997. Immunity. 7: 837-47. PMID: 9430229
  10. MyD88,一种参与白细胞介素-1 信号转导的适配蛋白。  |  Burns, K., et al. 1998. J Biol Chem. 273: 12203-9. PMID: 9575168
  11. MyD88基因的靶向干扰会导致IL-1和IL-18介导的功能丧失。  |  Adachi, O., et al. 1998. Immunity. 9: 143-50. PMID: 9697844
  12. MyD88是hToll/IL-1受体家族信号通路中的一种适配蛋白。  |  Medzhitov, R., et al. 1998. Mol Cell. 2: 253-8. PMID: 9734363

订购信息

产品名称产品编号规格价格数量收藏夹

MyD88 抗体 (E-11)

sc-74532
200 µg/ml
$316.00

MyD88 (E-11): m-IgG Fc BP-HRP 套装

sc-528685
200 µg Ab; 10 µg BP
$354.00

MyD88 (E-11): m-IgGκ BP-HRP 套装

sc-521134
200 µg Ab, 40 µg BP
$354.00

MyD88 (E-11): m-IgG2b BP-HRP 套装

sc-550065
200 µg Ab; 10 µg BP
$354.00

MyD88 抗体 (E-11) AC

sc-74532 AC
500 µg/ml, 25% agarose
$416.00

MyD88 抗体 (E-11) HRP

sc-74532 HRP
200 µg/ml
$316.00

MyD88 抗体 (E-11) FITC

sc-74532 FITC
200 µg/ml
$330.00

MyD88 抗体 (E-11) PE

sc-74532 PE
200 µg/ml
$343.00

MyD88 抗体 (E-11) Alexa Fluor® 488

sc-74532 AF488
200 µg/ml
$357.00

MyD88 抗体 (E-11) Alexa Fluor® 546

sc-74532 AF546
200 µg/ml
$357.00

MyD88 抗体 (E-11) Alexa Fluor® 594

sc-74532 AF594
200 µg/ml
$357.00

MyD88 抗体 (E-11) Alexa Fluor® 647

sc-74532 AF647
200 µg/ml
$357.00

MyD88 抗体 (E-11) Alexa Fluor® 680

sc-74532 AF680
200 µg/ml
$357.00

MyD88 抗体 (E-11) Alexa Fluor® 790

sc-74532 AF790
200 µg/ml
$357.00

I would like to get a free sample for MyD88 (E-11): sc-74532.

Asked by: MoAnn
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2020-04-14

What is the recommended secondary for WB?

Asked by: Anna99
We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2020-04-14
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Rated 5 out of 5 by from 特异性高购买该抗体,并用于蛋白免疫印迹实验。抗体特异性较高,根据组织中表达量不用,应根据情况适当调整整体上样量,已检测组间差异。
Date published: 2022-07-04
Rated 5 out of 5 by from excellentIt is easy to detect an expected band in A549 cells when use at 1/200X dilution for over night staining.
Date published: 2022-07-02
Rated 1 out of 5 by from Did not work for meUsed at a dilution of !:500 on mouse lung lysates for WB
Date published: 2021-02-18
Rated 4 out of 5 by from immunohistochemistry of myd88We can get a better result when we used this antibody, MyD88(E-11): sc-74532, in duodenum tissue.
Date published: 2020-09-24
Rated 5 out of 5 by from MyD88 (E-11)MyD88 (E-11) worked great for WB in mouse cell line.
Date published: 2020-03-12
Rated 4 out of 5 by from Good MyD88 antibody!This MyD88 antibody could work well in a dilution of 1: 500 , we got a very clear band, low background.
Date published: 2018-03-02
Rated 4 out of 5 by from Excellent Myd88 antibodyThis MyD88 antibody works very well using MCF-7 cell line.
Date published: 2018-02-27
Rated 1 out of 5 by from Very high backgroundI used it at two dilution 1:500 and 1:1000. Also my secondaries were from santa cruz as well. But the antibody gave me very very high background. I ended up buying from cell signaling which works perfect and gives you single band.
Date published: 2018-02-06
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MyD88 抗体 (E-11) is rated 4.2 out of 5 by 25.
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