MuRF1 Antibody (C-11): sc-398608

MuRF1 Antibody (C-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects MuRF1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). MuRF1 (C-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Muscle specific RING finger protein (MuRF1) plays a crucial role in muscle atrophy, being a sarcomere-associated protein that is upregulated in response to atrophic conditions. MuRF1 is essential for regulating the ubiquitin-proteasome pathway, which is responsible for protein degradation in muscle tissues. MuRF1 mediates the degradation of specific muscle proteins, highlighting its importance in maintaining muscle mass and function, particularly during stress conditions such as cancer, AIDS, and sepsis, where reactive oxygen species are elevated. MuRF1 interacts with Titin kinase, influencing microtubule-dependent signaling pathways in striated muscle and iris, thereby linking muscle structure to signaling mechanisms that govern hypertrophy and atrophy. The modulation of MuRF1 activity presents a potential therapeutic target for interventions aimed at reversing muscle wasting, making MuRF1 a significant focus in the study of muscle-related diseases and conditions.

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MuRF1 Antibody (C-11) References:

1. Partial kinetoplast-mitochondrial gene organization and expression in the respiratory deficient plant trypanosomatid Phytomonas serpens.  |  Maslov, DA., et al. 1999. Mol Biochem Parasitol. 99: 207-21. PMID: 10340485
2. Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain.  |  Centner, T., et al. 2001. J Mol Biol. 306: 717-26. PMID: 11243782
3. Identification of ubiquitin ligases required for skeletal muscle atrophy.  |  Bodine, SC., et al. 2001. Science. 294: 1704-8. PMID: 11679633
4. Sepsis upregulates the gene expression of multiple ubiquitin ligases in skeletal muscle.  |  Wray, CJ., et al. 2003. Int J Biochem Cell Biol. 35: 698-705. PMID: 12672461
5. [Epidemiologic aspects of whooping cough in a village of the central district of Kolokani in Mali].  |  Ag Bendech, M., et al. 1992. Bull Soc Pathol Exot. 85: 397-9. PMID: 1292803
6. Circadian rhythm of heart rate variability in survivors of cardiac arrest.  |  Huikuri, HV., et al. 1992. Am J Cardiol. 70: 610-5. PMID: 1510009
7. Muscle-specific RING finger 1 is a bona fide ubiquitin ligase that degrades cardiac troponin I.  |  Kedar, V., et al. 2004. Proc Natl Acad Sci U S A. 101: 18135-40. PMID: 15601779
8. MuRF1 activity is present in cardiac mitochondria and regulates reactive oxygen species production in vivo.  |  Mattox, TA., et al. 2014. J Bioenerg Biomembr. 46: 173-87. PMID: 24733503
9. The Calcineurin-FoxO-MuRF1 signaling pathway regulates myofibril integrity in cardiomyocytes.  |  Shimizu, H., et al. 2017. Elife. 6: PMID: 28826496
10. DCAF8, a novel MuRF1 interaction partner, promotes muscle atrophy.  |  Nowak, M., et al. 2019. J Cell Sci. 132: PMID: 31391242
11. Small-Molecule Inhibition of MuRF1 Prevents Early Disuse-Induced Diaphragmatic Dysfunction and Atrophy.  |  Ribeiro, F., et al. 2023. Int J Mol Sci. 24: PMID: 36835047
12. Murf1 alters myosin replacement rates in cultured myotubes in a myosin isoform-dependent manner.  |  Uenaka, E., et al. 2024. In Vitro Cell Dev Biol Anim. 60: 748-759. PMID: 38758432

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MuRF1 Antibody (C-11) has 107 citations in a variety of scientific publications.

Primary antibodies like Anti-MuRF1 Antibody (C-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MuRF1 Antibody (C-11) Specifications

Alternate Product Name: TRIM63

Product Clone Name: C-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 184-328

Epitope Target: MuRF1

Epitope Species: human

Product Reactivity/Detection: MuRF1 isoforms 1 and 2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 107 citations in various scientific publications

Available Conjugates/Forms for MuRF1 Antibody (C-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin