mtTFA Antibody (C-9): sc-376672

mtTFA Antibody (C-9) is a mouse monoclonal IgG2b kappa light chain antibody that specifically targets an epitope mapping between amino acids 207-241 near the C-terminus of human mtTFA, demonstrating reactivity in human samples via western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). mtTFA, also known as mitochondrial transcription factor A, TFAM, or TCF6, is a nuclear-encoded protein imported into the mitochondria, where mtTFA binds to mitochondrial DNA (mtDNA) and is crucial for the replication, transcription, and maintenance of mtDNA. As a member of the high mobility group (HMG) protein family, mtTFA has DNA-binding domains that allow mtTFA to bend and wrap mtDNA, facilitating the initiation of transcription and replication processes. The mitochondrial localization of mtTFA is vital because mtTFA directly influences mitochondrial gene expression and biogenesis, essential for proper mitochondrial function and energy production in cells. Aberrations in mtTFA expression or function can lead to mitochondrial dysfunction and are associated with various mitochondrial disorders characterized by symptoms like muscle weakness, neurodegeneration, and metabolic issues. During spermatogenesis, mtTFA levels decrease, leading to reduced mtDNA copy number, which may prevent the transmission of paternal mtDNA. Understanding mtTFA′s role in mitochondrial health is important for research into aging, metabolic diseases, and mitochondrial pathologies. anti-mtTFA antibody (C-9) is available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatility for diverse research applications involving mitochondrial function and related diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

mtTFA Antibody (C-9) References:

1. Abnormal H-Tfam in a patient harboring a single mtDNA deletion.  |  Tessa, A., et al. 2000. Funct Neurol. 15: 211-4. PMID: 11213524
2. Abnormal levels of human mitochondrial transcription factor A in skeletal muscle in mitochondrial encephalomyopathies.  |  Siciliano, G., et al. 2000. Neurol Sci. 21: S985-7. PMID: 11382203
3. Mitochondrial transcription factor A (mtTFA) and diabetes.  |  Choi, YS., et al. 2001. Diabetes Res Clin Pract. 54 Suppl 2: S3-9. PMID: 11733104
4. Mitochondrial transcription factor A (TFAM) is upregulated in glioma.  |  Lee, H., et al. 2017. Mol Med Rep. 15: 3781-3786. PMID: 28440425
5. Mitochondrial transcription factor A (TFAM) shapes metabolic and invasion gene signatures in melanoma.  |  Araujo, LF., et al. 2018. Sci Rep. 8: 14190. PMID: 30242167
6. Heat shock protein 22 modulates NRF1/TFAM-dependent mitochondrial biogenesis and DRP1-sparked mitochondrial apoptosis through AMPK-PGC1α signaling pathway to alleviate the early brain injury of subarachnoid hemorrhage in rats.  |  Fan, H., et al. 2021. Redox Biol. 40: 101856. PMID: 33472123
7. TFAM deficiency in dendritic cells leads to mitochondrial dysfunction and enhanced antitumor immunity through cGAS-STING pathway.  |  Lu, T., et al. 2023. J Immunother Cancer. 11: PMID: 36858460
8. Astragaloside IV attenuates podocyte apoptosis through ameliorating mitochondrial dysfunction by up-regulated Nrf2-ARE/TFAM signaling in diabetic kidney disease.  |  Shen, Q., et al. 2023. Free Radic Biol Med. 203: 45-57. PMID: 37030337
9. Mitochondrial transcription factor A (TFAM) has 5'-deoxyribose phosphate lyase activity in vitro.  |  Zhao, W., et al. 2024. DNA Repair (Amst). 137: 103666. PMID: 38492429
10. Chromosomal localization of mitochondrial transcription factor A (TCF6), single-stranded DNA-binding protein (SSBP), and endonuclease G (ENDOG), three human housekeeping genes involved in mitochondrial biogenesis.  |  Tiranti, V., et al. 1995. Genomics. 25: 559-64. PMID: 7789991
11. A testis-specific gene encoding a nuclear high-mobility-group box protein located in elongating spermatids.  |  Boissonneault, G. and Lau, YF. 1993. Mol Cell Biol. 13: 4323-30. PMID: 8321234
12. A single mouse gene encodes the mitochondrial transcription factor A and a testis-specific nuclear HMG-box protein.  |  Larsson, NG., et al. 1996. Nat Genet. 13: 296-302. PMID: 8673128

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-mtTFA Antibody (C-9) has 29 citations in a variety of scientific publications.

Primary antibodies like Anti-mtTFA Antibody (C-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

mtTFA Antibody (C-9) Specifications

Alternate Product Name: mtTFA

Product Clone Name: C-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 207-241

Epitope Target: mtTFA

Epitope Species: human

Product Reactivity/Detection: mtTFA

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 29 citations in various scientific publications

Available Conjugates/Forms for mtTFA Antibody (C-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred mtTFA antibody is mtTFA Antibody (F-6): sc-166965.