mTOR Antibody (30): sc-517464

mTOR Antibody (30) is a mouse monoclonal IgG1 lambda light chain antibody engineered to detect the mechanistic target of rapamycin (mTOR) protein, recognizing the amino acid region 185-290 of rat origin. mTOR, also called SKS, FRAP, FRAP1, FRAP2, RAFT1, and RAPT1, is a pivotal serine/threonine kinase that plays a critical role in regulating cell growth, proliferation, metabolism, and autophagy by integrating signals from nutrients, growth factors, and cellular energy status. Located primarily in the cytoplasm, mTOR functions within two distinct complexes, mTORC1 and mTORC2, each orchestrating different cellular processes essential for maintaining cellular homeostasis and responding to environmental cues. The structure of mTOR, featuring a conserved kinase domain and multiple regulatory regions, is fundamental for its ability to phosphorylate downstream targets such as S6 kinase and 4E-BP1, thereby controlling protein synthesis and cell cycle progression. Post-translational modifications, including autophosphorylation at Ser2481 and phosphorylation at Ser2448 by p70S6 kinase, are crucial for regulating mTOR activity and its interactions with other proteins. mTOR interacts with a variety of proteins, including Raptor and Rictor, which are integral components of mTORC1 and mTORC2, respectively, as well as with regulatory proteins like TSC1/2 and AMPK that modulate its activity in response to cellular stress and nutrient availability. These interactions and modifications are essential for mTOR′s role in cellular signaling pathways that influence growth and survival, making mTOR a vital target for research in cancer, neurodegeneration, and metabolic diseases. Anti-mTOR antibody (30) is validated for use in western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) across mouse, rat, and human samples, ensuring broad applicability in various experimental settings. Additionally, mTOR (30) antibody is available in both non-conjugated form and conjugated forms, including agarose and horseradish peroxidase (HRP), providing flexibility for diverse laboratory applications and facilitating the detection and study of mTOR in intricate cellular environments.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

mTOR Antibody (30) References:

1. mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery.  |  Kim, DH., et al. 2002. Cell. 110: 163-75. PMID: 12150925
2. mTOR signalling and cellular metabolism are mutual determinants in cancer.  |  Mossmann, D., et al. 2018. Nat Rev Cancer. 18: 744-757. PMID: 30425336
3. The mTOR-Autophagy Axis and the Control of Metabolism.  |  Deleyto-Seldas, N. and Efeyan, A. 2021. Front Cell Dev Biol. 9: 655731. PMID: 34277603
4. The Translational Regulation in mTOR Pathway.  |  Yang, M., et al. 2022. Biomolecules. 12: PMID: 35740927
5. Overview of Research into mTOR Inhibitors.  |  Mao, B., et al. 2022. Molecules. 27: PMID: 36014530
6. Targeting mTOR for Anti-Aging and Anti-Cancer Therapy.  |  Fu, W. and Wu, G. 2023. Molecules. 28: PMID: 37049920
7. mTOR in programmed cell death and its therapeutic implications.  |  Xie, Y., et al. 2023. Cytokine Growth Factor Rev. 71-72: 66-81. PMID: 37380596
8. When is a lipid kinase not a lipid kinase? When it is a protein kinase.  |  Hunter, T. 1995. Cell. 83: 1-4. PMID: 7553860
9. PIK-related kinases: DNA repair, recombination, and cell cycle checkpoints.  |  Keith, CT. and Schreiber, SL. 1995. Science. 270: 50-1. PMID: 7569949
10. DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product.  |  Hartley, KO., et al. 1995. Cell. 82: 849-56. PMID: 7671312
11. Discovery of AT gene sparks biomedical research bonanza.  |  Nowak, R. 1995. Science. 268: 1700-1. PMID: 7792589
12. A single ataxia telangiectasia gene with a product similar to PI-3 kinase.  |  Savitsky, K., et al. 1995. Science. 268: 1749-53. PMID: 7792600

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-mTOR Antibody (30) has 121 citations in a variety of scientific publications.

Primary antibodies like Anti-mTOR Antibody (30) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

mTOR Antibody (30) Specifications

Alternate Product Name: mTOR

Product Clone Name: 30

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ lambda light chain

Amino Acids: 185-290

Epitope Target: mTOR

Epitope Species: rat

Product Reactivity/Detection: mTOR

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ lambda light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 121 citations in various scientific publications

Available Conjugates/Forms for mTOR Antibody (30): AC: Agarose, HRP: Horseradish Peroxidase

The preferred p-mTOR antibody is p-mTOR Antibody (59.Ser 2448): sc-293133.