Anti-MTHFD1L Antibody (D-7) recommended for detection of MTHFD1L of human origin by WB, IP, IF and ELISA

Anti-MTHFD1L Antibody (D-7): sc-515706

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Datasheets

    • MTHFD1L Antibody (D-7) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 73-178 mapping near the N-terminus of MTHFD1L of human origin
    • recommended for detection of MTHFD1L of human origin by WB, IP, IF and ELISA
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MTHFD1L Antibody (D-7) for WB applications. These reagents are now offered in bundles with MTHFD1L Antibody (D-7) (see ordering information below).
loading

How much sample do you recommend loading on the gel for Western blot detection using MTHFD1L (D-7): sc-515706?

Asked by: jerojero
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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