MTHFD1/1L Antibody (D-9): sc-376722

MTHFD1/1L Antibody (D-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects MTHFD1/1L protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MTHFD1/1L (D-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MTHFD1 protein, consisting of 935 amino acids, plays a crucial role in folate metabolism by facilitating the interconversion of tetrahydrofolate derivatives, which are essential for the synthesis of purines, methionine, and thymidylate. MTHFD1 is located in the cytosol and functions as a homodimer, comprising three distinct subunits that contribute to enzymatic activities: 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase, and 10-formyltetrahydrofolate synthetase. Proper functioning of MTHFD1 is vital, as MTHFD1 not only supports cellular proliferation and growth but also has implications in developmental processes; mutations in the MTHFD1 gene in pregnant women have been linked to an increased risk of neural tube defects in offspring. Additionally, MTHFD1L, a related mitochondrial protein of 978 amino acids, is expressed in various tissues and is involved in similar metabolic pathways, with potential associations to colorectal cancer progression. MTHFD1/1L (D-9) monoclonal antibody′s availability in multiple formats makes this reagent a versatile tool for researchers studying folate metabolism and its implications in health and disease.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MTHFD1/1L Antibody (D-9) References:

1. Role of polymorphisms in MTHFR and MTHFD1 genes in the outcome of childhood acute lymphoblastic leukemia.  |  Krajinovic, M., et al. 2004. Pharmacogenomics J. 4: 66-72. PMID: 14647408
2. Disruption of the mthfd1 gene reveals a monofunctional 10-formyltetrahydrofolate synthetase in mammalian mitochondria.  |  Christensen, KE., et al. 2005. J Biol Chem. 280: 7597-602. PMID: 15611115
3. MTHFD1 R653Q polymorphism is a maternal genetic risk factor for severe abruptio placentae.  |  Parle-McDermott, A., et al. 2005. Am J Med Genet A. 132A: 365-8. PMID: 15633187
4. Folate-related genes and omphalocele.  |  Mills, JL., et al. 2005. Am J Med Genet A. 136: 8-11. PMID: 15937947
5. Thymidylate synthase promoter tandem repeat and MTHFD1 R653Q polymorphisms modulate the risk for migraine conferred by the MTHFR T677 allele.  |  Oterino, A., et al. 2005. Brain Res Mol Brain Res. 139: 163-8. PMID: 15953655
6. A polymorphism in the MTHFD1 gene increases a mother's risk of having an unexplained second trimester pregnancy loss.  |  Parle-McDermott, A., et al. 2005. Mol Hum Reprod. 11: 477-80. PMID: 16123074
7. Confirmation of the R653Q polymorphism of the trifunctional C1-synthase enzyme as a maternal risk for neural tube defects in the Irish population.  |  Parle-McDermott, A., et al. 2006. Eur J Hum Genet. 14: 768-72. PMID: 16552426
8. B vitamin treatments modify the risk of myocardial infarction associated with a MTHFD1 polymorphism in patients with stable angina pectoris.  |  Ding, YP., et al. 2016. Nutr Metab Cardiovasc Dis. 26: 495-501. PMID: 26803590
9. MTHFD1 regulates nuclear de novo thymidylate biosynthesis and genome stability.  |  Field, MS., et al. 2016. Biochimie. 126: 27-30. PMID: 26853819
10. Association of Maternal Dietary Habits and MTHFD1 Gene Polymorphisms With Ventricular Septal Defects in Offspring: A Case-Control Study.  |  Song, X., et al. 2021. Front Pediatr. 9: 785440. PMID: 35186819
11. A Common Polymorphism in the MTHFD1 Gene Is a Modulator of Risk of Congenital Heart Disease.  |  Karas Kuželički, N., et al. 2022. J Cardiovasc Dev Dis. 9: PMID: 35735795
12. Congenital defects in folate utilization.  |  Arakawa, T. 1970. Am J Med. 48: 594-8. PMID: 5425237

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MTHFD1/1L Antibody (D-9) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-MTHFD1/1L Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MTHFD1/1L Antibody (D-9) Specifications

Alternate Product Name: MTHFD1/1L

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 909-935

Epitope Target: MTHFD1

Epitope Species: human

Product Reactivity/Detection: MTHFD1 and MTHFD1L

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for MTHFD1/1L Antibody (D-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin