mSin3B Antibody (H-5): sc-55516

mSin3B Antibody (H-5) is a mouse monoclonal IgG2b antibody that detects mSin3B in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-mSin3B antibody (H-5) plays a crucial role in the regulation of gene expression by interacting with the Mad family of proteins, which are essential for transcriptional repression. mSin3B (H-5) antibody is primarily located in the nucleus, where mSin3B functions as a corepressor by forming complexes with Mad-Max heterodimers. This interaction is vital for the repression of target genes involved in cell proliferation and differentiation, thereby influencing various cellular processes and maintaining cellular homeostasis. mSin3B monoclonal antibody (H-5) targets a protein that effectively modulates transcriptional activity in response to cellular signals, making mSin3B an important player in the regulation of developmental processes and tumorigenesis. mSin3B′s ability to interact with multiple transcription factors highlights its significance in the intricate network of gene regulation, and mSin3B′s stable expression contrasts with the short half-lives of its binding partners, ensuring a consistent regulatory mechanism.

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mSin3B Antibody (H-5) References:

1. Neural restrictive silencer factor recruits mSin3 and histone deacetylase complex to repress neuron-specific target genes.  |  Naruse, Y., et al. 1999. Proc Natl Acad Sci U S A. 96: 13691-6. PMID: 10570134
2. Expression of transcriptional repressor proteins mSin3A and 3B during aging and replicative senescence.  |  Kyrylenko, S., et al. 2000. Biochem Biophys Res Commun. 275: 455-9. PMID: 10964686
3. Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex.  |  Alland, L., et al. 2002. Mol Cell Biol. 22: 2743-50. PMID: 11909966
4. An ERG (ets-related gene)-associated histone methyltransferase interacts with histone deacetylases 1/2 and transcription co-repressors mSin3A/B.  |  Yang, L., et al. 2003. Biochem J. 369: 651-7. PMID: 12398767
5. Role of structural and dynamical plasticity in Sin3: the free PAH2 domain is a folded module in mSin3B.  |  van Ingen, H., et al. 2006. J Mol Biol. 358: 485-97. PMID: 16519900
6. Coupled unfolding and dimerization by the PAH2 domain of the mammalian Sin3A corepressor.  |  Zhang, Y., et al. 2006. J Mol Biol. 360: 7-14. PMID: 16813833
7. SWI/SNF activity is required for the repression of deoxyribonucleotide triphosphate metabolic enzymes via the recruitment of mSin3B.  |  Gunawardena, RW., et al. 2007. J Biol Chem. 282: 20116-23. PMID: 17510060
8. Solution NMR studies of apo-mSin3A and -mSin3B reveal that the PAH1 and PAH2 domains are structurally independent.  |  He, Y. and Radhakrishnan, I. 2008. Protein Sci. 17: 171-5. PMID: 18042683
9. Binding of the repressor complex REST-mSIN3b by small molecules restores neuronal gene transcription in Huntington's disease models.  |  Conforti, P., et al. 2013. J Neurochem. 127: 22-35. PMID: 23800350
10. Expression of transcriptional repressor protein mSin3A but not mSin3B is induced during neuronal apoptosis.  |  Korhonen, P., et al. 1998. Biochem Biophys Res Commun. 252: 274-7. PMID: 9813182

The preferred mSin3B antibody is mSin3B Antibody (H-4): sc-13145.