mSin3B Antibody (H-4): sc-13145

mSin3B Antibody (H-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects mSin3B of mouse, rat, and human origin through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). mSin3B (H-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. mSin3B plays a crucial role in the regulation of gene expression, particularly in cell proliferation and differentiation, by functioning as a corepressor in transcriptional regulation. mSin3B belongs to a larger family of proteins that interact with transcription factors such as Myc and Mad proteins, which are essential for maintaining cellular homeostasis. mSin3B interaction with these transcription factors helps modulate the expression of genes involved in critical cellular processes, including growth and apoptosis. mSin3B interacts with Mad proteins through paired amphipathic helix domains, facilitating recruitment of additional corepressors to target genes, thereby influencing transcriptional repression. This regulatory mechanism underscores mSin3B importance in maintaining balance between cell proliferation and differentiation, making mSin3B a significant player in cellular signaling pathways.

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mSin3B Antibody (H-4) References:

1. E2F mediates cell cycle-dependent transcriptional repression in vivo by recruitment of an HDAC1/mSin3B corepressor complex.  |  Rayman, JB., et al. 2002. Genes Dev. 16: 933-47. PMID: 11959842
2. Inhibition of polyomavirus ori-dependent DNA replication by mSin3B.  |  Xie, AY. and Folk, WR. 2002. J Virol. 76: 11809-18. PMID: 12414923
3. Myc and Max proteins possess distinct transcriptional activities.  |  Kretzner, L., et al. 1992. Nature. 359: 426-9. PMID: 1406956
4. Myc family oncoproteins function through a common pathway to transform normal cells in culture: cross-interference by Max and trans-acting dominant mutants.  |  Mukherjee, B., et al. 1992. Genes Dev. 6: 1480-92. PMID: 1644290
5. Specific requirement of the chromatin modifier mSin3B in cell cycle exit and cellular differentiation.  |  David, G., et al. 2008. Proc Natl Acad Sci U S A. 105: 4168-72. PMID: 18332431
6. Difference of binding modes among three ligands to a receptor mSin3B corresponding to their inhibitory activities.  |  Hayami, T., et al. 2021. Sci Rep. 11: 6178. PMID: 33731831
7. Generalized-ensemble method study: A helix-mimetic compound inhibits protein-protein interaction by long-range and short-range intermolecular interactions.  |  Higo, J., et al. 2021. J Comput Chem. 42: 956-969. PMID: 33755222
8. Mad-Max transcriptional repression is mediated by ternary complex formation with mammalian homologs of yeast repressor Sin3.  |  Ayer, DE., et al. 1995. Cell. 80: 767-76. PMID: 7889570
9. An amino-terminal domain of Mxi1 mediates anti-Myc oncogenic activity and interacts with a homolog of the yeast transcriptional repressor SIN3.  |  Schreiber-Agus, N., et al. 1995. Cell. 80: 777-86. PMID: 7889571
10. The c-Myc protein induces cell cycle progression and apoptosis through dimerization with Max.  |  Amati, B., et al. 1993. EMBO J. 12: 5083-7. PMID: 8262051
11. Mad: a heterodimeric partner for Max that antagonizes Myc transcriptional activity.  |  Ayer, DE., et al. 1993. Cell. 72: 211-22. PMID: 8425218
12. Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation.  |  Hurlin, PJ., et al. 1995. EMBO J. 14: 5646-59. PMID: 8521822

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-mSin3B Antibody (H-4) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-mSin3B Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

mSin3B Antibody (H-4) Specifications

Alternate Product Name: mSin3B

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 172-228

Epitope Target: mSin3B

Epitope Species: mouse

Product Reactivity/Detection: mSin3B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for mSin3B Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent