MRTF-A/MKL1 Antibody (G-8): sc-390324

MRTF-A Antibody (G-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects MRTF-A in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-MRTF-A antibody (G-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MRTF-A, also known as myocardin-related transcription factor A, plays a crucial role in regulating gene expression in response to growth factors, particularly in muscle cells. MRTF-A primarily localizes in the nucleus, where MRTF-A interacts with serum response factor (SRF) to enhance transcriptional activity of muscle-specific genes. This nuclear localization enables MRTF-A to effectively modulate the expression of genes involved in muscle development and cardiovascular function, influencing processes such as myogenesis and vascular smooth muscle differentiation. MRTF-A and SRF interaction is essential for cardiovascular system formation during vertebrate development, highlighting MRTF-A′s importance in both normal physiological processes and potential pathological conditions.

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MRTF-A/MKL1 Antibody (G-8) References:

1. Potentiation of serum response factor activity by a family of myocardin-related transcription factors.  |  Wang, DZ., et al. 2002. Proc Natl Acad Sci U S A. 99: 14855-60. PMID: 12397177
2. Sphingosine 1-phosphate stimulates smooth muscle cell differentiation and proliferation by activating separate serum response factor co-factors.  |  Lockman, K., et al. 2004. J Biol Chem. 279: 42422-30. PMID: 15292266
3. Identification of direct serum-response factor gene targets during Me2SO-induced P19 cardiac cell differentiation.  |  Zhang, SX., et al. 2005. J Biol Chem. 280: 19115-26. PMID: 15699019
4. Acute myeloid leukemia-associated Mkl1 (Mrtf-a) is a key regulator of mammary gland function.  |  Sun, Y., et al. 2006. Mol Cell Biol. 26: 5809-26. PMID: 16847333
5. The SRE: a growth factor responsive transcriptional regulator.  |  Treisman, R. 1990. Semin Cancer Biol. 1: 47-58. PMID: 2133110
6. The role of the MRTF-A/SRF pathway in ocular fibrosis.  |  Yu-Wai-Man, C., et al. 2014. Invest Ophthalmol Vis Sci. 55: 4560-7. PMID: 25056592
7. Isolation and properties of cDNA clones encoding SRF, a transcription factor that binds to the c-fos serum response element.  |  Norman, C., et al. 1988. Cell. 55: 989-1003. PMID: 3203386
8. Actin-microtubule cytoskeletal interplay mediated by MRTF-A/SRF signaling promotes dilated cardiomyopathy caused by LMNA mutations.  |  Le Dour, C., et al. 2022. Nat Commun. 13: 7886. PMID: 36550158
9. An MRTF-A-ZEB1-IRF9 axis contributes to fibroblast-myofibroblast transition and renal fibrosis.  |  Zhao, Q., et al. 2023. Exp Mol Med. 55: 987-998. PMID: 37121967
10. MRTF-A gain-of-function in mice impairs homeostatic renewal of the intestinal epithelium.  |  Singh, AK., et al. 2023. Cell Death Dis. 14: 639. PMID: 37770456
11. Gas6-Axl Signaling Induces SRF/MRTF-A Gene Transcription via MICAL2.  |  Lundquist, MR. and Jaffrey, SR. 2023. Genes (Basel). 14: PMID: 38137053
12. Functional analysis of a growth factor-responsive transcription factor complex.  |  Hill, CS., et al. 1993. Cell. 73: 395-406. PMID: 8477450

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MRTF-A/MKL1 Antibody (G-8) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-MRTF-A/MKL1 Antibody (G-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MRTF-A/MKL1 Antibody (G-8) Specifications

Alternate Product Name: MRTF-A

Product Clone Name: G-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 761-900

Epitope Target: MRTF-A

Epitope Species: human

Product Reactivity/Detection: MRTF-A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for MRTF-A/MKL1 Antibody (G-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred MRTF-A/MKL1 antibody is MRTF-A/MKL1 Antibody (G-7): sc-398675.