MYL12A/B Antibody (G-5): sc-376677
- MYL12A/B Antibody (G-5) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 103-141 within an internal region of MYL12A of human origin
- recommended for detection of the myosin regulatory light chains encoded by MYL12A and MYL12B of human origin, Mylc2b of mouse origin and Mrlcb of rat origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- See MYL9/MYL12A/B (E-4): sc-28329 for MYL12A/B antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MYL12A/B Antibody (G-5) for WB applications. These reagents are now offered in bundles with MYL12A/B Antibody (G-5) (see ordering information below).
MYL12A/B Antibody (G-5) is a mouse monoclonal IgG1 antibody that detects MYL12A in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MYL12A, also known as MRLC3 or MLCB, plays a crucial role in regulating contraction in smooth muscle and non-muscle cells by undergoing phosphorylation through the action of myosin light chain kinase (MLCK) in the presence of calcium and calmodulin. This post-translational modification is vital as it enhances the Actin-activated myosin ATPase activity, thereby modulating the contractile function of these cells. The phosphorylation state of MYL12A is essential for various physiological processes, including muscle contraction and cellular motility, making MYL12A a key player in both normal and pathological conditions. Additionally, MYL12A is found in striated skeletal muscle, where MYL12A′s precise function remains to be fully elucidated, highlighting the importance of further research into MYL12A′s role in muscle physiology. MYL12A (G-5) antibody serves as an invaluable tool for researchers investigating the dynamics of muscle contraction and the regulatory mechanisms underlying cellular movement.
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LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MYL12A/B Antibody (G-5) References:
- Chimeric myosin regulatory light chains identify the subdomain responsible for regulatory function. | Rowe, T. and Kendrick-Jones, J. 1992. EMBO J. 11: 4715-22. PMID: 1464306
- The significance of the slow dissociation of divalent metal ions from myosin 'regulatory' light chains. | Bagshaw, CR. and Reed, GH. 1977. FEBS Lett. 81: 386-90. PMID: 200479
- Mammalian nonsarcomeric myosin regulatory light chains are encoded by two differentially regulated and linked genes. | Grant, JW., et al. 1990. J Cell Biol. 111: 1127-35. PMID: 2391362
- Functional and Molecular Characterisation of Heart Failure Progression in Mice and the Role of Myosin Regulatory Light Chains in the Recovery of Cardiac Muscle Function. | Markandran, K., et al. 2021. Int J Mol Sci. 23: PMID: 35008512
- Phosphorylation of myosin regulatory light chains by the molluscan twitchin kinase. | Heierhorst, J., et al. 1995. Eur J Biochem. 233: 426-31. PMID: 7588784
- Skeletal muscle myosin regulatory light chains conformation affects the papain cleavage of A1 light chains. | Stepkowski, D., et al. 1994. Biochim Biophys Acta. 1209: 253-9. PMID: 7811699
- Fluorescent probes of the orientation of myosin regulatory light chains in relaxed, rigor, and contracting muscle. | Ling, N., et al. 1996. Biophys J. 70: 1836-46. PMID: 8785344
- Orientation changes in myosin regulatory light chains following photorelease of ATP in skinned muscle fibers. | Allen, TS., et al. 1996. Biophys J. 70: 1847-62. PMID: 8785345
- Structural and functional responses of mammalian thick filaments to alterations in myosin regulatory light chains. | Levine, RJ., et al. 1998. J Struct Biol. 122: 149-61. PMID: 9724616
- Calcium binding regions of myosin 'regulatory' light chains. | Jakes, R., et al. 1976. FEBS Lett. 70: 229-34. PMID: 992065
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MYL12A/B Antibody (G-5) | sc-376677 | 200 µg/ml | $322.00 | |||
MYL12A/B Antibody (G-5): m-IgG Fc BP-HRP Bundle | sc-537998 | 200 µg Ab; 10 µg BP | $361.00 | |||
MYL12A/B Antibody (G-5): m-IgGκ BP-HRP Bundle | sc-535504 | 200 µg Ab; 40 µg BP | $361.00 | |||
MYL12A/B Antibody (G-5): m-IgG1 BP-HRP Bundle | sc-545482 | 200 µg Ab; 20 µg BP | $361.00 | |||
MRCL3/MRLC2 (G-5) Neutralizing Peptide | sc-376677 P | 100 µg/0.5 ml | $69.00 |