MYL12A/B Antibody (A-10): sc-376606
- MYL12A/B Antibody (A-10) is a mouse monoclonal IgG2b κ, cited in 4 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 103-141 within an internal region of MYL12A of human origin
- MYL12A/B Antibody (A-10) is recommended for detection of the myosin regulatory light chains encoded by MYL12A and MYL12B of human origin, Mylc2b of mouse origin and Mrlcb of rat origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-MYL12A/B Antibody (A-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for MYL12A/B Antibody (A-10) for WB and IHC(P) applications. These reagents are now offered in bundles with MYL12A/B Antibody (A-10) (see ordering information below).
MYL12A/B Antibody (A-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects the myosin regulatory light chains encoded by MYL12A and MYL12B of human origin, Mylc2b of mouse origin, and Mrlcb of rat origin, demonstrating reactivity across mouse, rat, and human species through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). MYL12A/B (A-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Myosin regulatory light chains, including MYL12A (also known as MRLC3 or MLCB) and MYL12B (also known as MRLC2), play a crucial role in muscle contraction and cellular motility by regulating the interaction between myosin and actin filaments. These light chains undergo phosphorylation by myosin light chain kinase (MLCK) in the presence of calcium and calmodulin, which enhances the actin-activated myosin ATPase activity, thereby modulating contractile activity in smooth muscle and non-muscle cells. The phosphorylation state of these light chains is vital for proper muscle function, as phosphorylation directly influences the contractile force generated during muscle contraction. Additionally, myosin light chains are also present in striated skeletal muscle, where specific roles are still being elucidated, highlighting the value of anti-MYL12A/B antibody (A-10) in studying muscle physiology and related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MYL12A/B Antibody (A-10) References:
- Inhibition of Rho-associated kinase blocks agonist-induced Ca2+ sensitization of myosin phosphorylation and force in guinea-pig ileum. | Swärd, K., et al. 2000. J Physiol. 522 Pt 1: 33-49. PMID: 10618150
- Functional role of the C-terminal domain of smooth muscle myosin light chain kinase on the phosphorylation of smooth muscle myosin. | Numata, T., et al. 2001. J Biochem. 129: 437-44. PMID: 11226884
- Rho kinase mediates serum-induced contraction in fibroblast fibers independent of myosin LC20 phosphorylation. | Nobe, H., et al. 2003. Am J Physiol Cell Physiol. 284: C599-606. PMID: 12388108
- ERK-mediated uterine artery contraction: role of thick and thin filament regulatory pathways. | Xiao, D., et al. 2004. Am J Physiol Heart Circ Physiol. 286: H1615-22. PMID: 15072969
- The E22K mutation of myosin RLC that causes familial hypertrophic cardiomyopathy increases calcium sensitivity of force and ATPase in transgenic mice. | Szczesna-Cordary, D., et al. 2005. J Cell Sci. 118: 3675-83. PMID: 16076902
- Integrin-linked kinase is responsible for Ca2+-independent myosin diphosphorylation and contraction of vascular smooth muscle. | Wilson, DP., et al. 2005. Biochem J. 392: 641-8. PMID: 16201970
- Smooth muscle myosin isoform expression and LC20 phosphorylation in innate rat airway hyperresponsiveness. | Gil, FR., et al. 2006. Am J Physiol Lung Cell Mol Physiol. 291: L932-40. PMID: 16766577
- Myosin regulatory light chains are required to maintain the stability of myosin II and cellular integrity. | Park, I., et al. 2011. Biochem J. 434: 171-80. PMID: 21126233
- Characterization and differential expression of human vascular smooth muscle myosin light chain 2 isoform in nonmuscle cells. | Kumar, CC., et al. 1989. Biochemistry. 28: 4027-35. PMID: 2526655
- Identification of phosphorylated MYL12B as a potential plasma biomarker for septic acute kidney injury using a quantitative proteomic approach. | Wu, F., et al. 2015. Int J Clin Exp Pathol. 8: 14409-16. PMID: 26823757
- Rupatadine-inhibited OTUD3 promotes DLBCL progression and immune evasion through deubiquitinating MYL12A and PD-L1. | Sui, Y., et al. 2024. Cell Death Dis. 15: 561. PMID: 39097608
- Ca2+-independent myosin II phosphorylation and contraction in chicken embryo fibroblasts. | Kolodney, MS., et al. 1999. J Physiol. 515 (Pt 1): 87-92. PMID: 9925880
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MYL12A/B Antibody (A-10) | sc-376606 | 200 µg/ml | $316.00 | |||
MYL12A/B Antibody (A-10): m-IgG Fc BP-HRP Bundle | sc-526039 | 200 µg Ab; 10 µg BP | $354.00 | |||
MYL12A/B Antibody (A-10): m-IgG2b BP-HRP Bundle | sc-549666 | 200 µg Ab; 10 µg BP | $354.00 | |||
MYL12A/B Antibody (A-10) AC | sc-376606 AC | 500 µg/ml, 25% agarose | $416.00 | |||
MYL12A/B Antibody (A-10) HRP | sc-376606 HRP | 200 µg/ml | $316.00 | |||
MYL12A/B Antibody (A-10) FITC | sc-376606 FITC | 200 µg/ml | $330.00 | |||
MYL12A/B Antibody (A-10) PE | sc-376606 PE | 200 µg/ml | $343.00 | |||
MYL12A/B Antibody (A-10) Alexa Fluor® 488 | sc-376606 AF488 | 200 µg/ml | $357.00 | |||
MYL12A/B Antibody (A-10) Alexa Fluor® 546 | sc-376606 AF546 | 200 µg/ml | $357.00 | |||
MYL12A/B Antibody (A-10) Alexa Fluor® 594 | sc-376606 AF594 | 200 µg/ml | $357.00 | |||
MYL12A/B Antibody (A-10) Alexa Fluor® 647 | sc-376606 AF647 | 200 µg/ml | $357.00 | |||
MYL12A/B Antibody (A-10) Alexa Fluor® 680 | sc-376606 AF680 | 200 µg/ml | $357.00 | |||
MYL12A/B Antibody (A-10) Alexa Fluor® 790 | sc-376606 AF790 | 200 µg/ml | $357.00 | |||
MYL12A/B (A-10) Neutralizing Peptide | sc-376606 P | 100 µg/0.5 ml | $68.00 |