MRCKβ Antibody (C-12): sc-374597
- MRCKβ Antibody (C-12) is a mouse monoclonal IgG1 κ MRCKβ antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 916-1040 mapping within an internal region of MRCKβ of human origin
- MRCK beta Antibody (C-12) is recommended for detection of MRCKβ of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-MRCK beta Antibody (C-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for MRCKβ Antibody (C-12) for WB applications. These reagents are now offered in bundles with MRCKβ Antibody (C-12) (see ordering information below).
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MRCKβ Antibody (C-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects MRCK beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-MRCKβ antibody (C-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MRCK beta protein plays a crucial role in cellular signaling pathways, particularly in regulating actin dynamics and cell motility. MRCK beta protein is primarily located in the cytoplasm, where MRCK beta protein interacts with nonmuscle myosin light chains and influences actin-myosin contractility, which is essential for various cellular processes such as migration, division, and shape maintenance. MRCK beta protein′s ability to phosphorylate and activate downstream targets, including LIM kinases, underscores MRCK beta protein′s importance in cytoskeletal reorganization and neurite outgrowth, making MRCK beta protein a significant player in both normal physiological functions and pathological conditions. MRCKβ (C-12) antibody can help researchers gain valuable insights into mechanisms of cell movement and underlying processes of diseases related to cytoskeletal dysfunction.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MRCKβ Antibody (C-12) References:
- Cloning and chromosomal localization of human Cdc42-binding protein kinase beta. | Moncrieff, CL., et al. 1999. Genomics. 57: 297-300. PMID: 10198171
- The myotonic dystrophy kinase-related Cdc42-binding kinase is involved in the regulation of neurite outgrowth in PC12 cells. | Chen, XQ., et al. 1999. J Biol Chem. 274: 19901-5. PMID: 10391936
- Signaling--2000 and beyond. | Hunter, T. 2000. Cell. 100: 113-27. PMID: 10647936
- Activation of LIM kinases by myotonic dystrophy kinase-related Cdc42-binding kinase alpha. | Sumi, T., et al. 2001. J Biol Chem. 276: 23092-6. PMID: 11340065
- Cdc42-dependent formation of the ZO-1/MRCKβ complex at the leading edge controls cell migration. | Huo, L., et al. 2011. EMBO J. 30: 665-78. PMID: 21240187
- Co-crystal structures of inhibitors with MRCKβ, a key regulator of tumor cell invasion. | Heikkila, T., et al. 2011. PLoS One. 6: e24825. PMID: 21949762
- A novel small-molecule MRCK inhibitor blocks cancer cell invasion. | Unbekandt, M., et al. 2014. Cell Commun Signal. 12: 54. PMID: 25288205
- Discovery of Potent and Selective MRCK Inhibitors with Therapeutic Effect on Skin Cancer. | Unbekandt, M., et al. 2018. Cancer Res. 78: 2096-2114. PMID: 29382705
- Identification of Kinases and Interactors of p53 Using Kinase-Catalyzed Cross-Linking and Immunoprecipitation. | Garre, S., et al. 2018. J Am Chem Soc. 140: 16299-16310. PMID: 30339384
- Moxifloxacin induces random migration in human corneal fibroblasts via the protein kinase C epsilon/zonula occludens-1 signaling pathway. | Chen, TC. and Chang, SW. 2021. Eur J Pharmacol. 910: 174414. PMID: 34425101
- Protein kinases and phosphatases: the yin and yang of protein phosphorylation and signaling. | Hunter, T. 1995. Cell. 80: 225-36. PMID: 7834742
- Myotonic dystrophy kinase-related Cdc42-binding kinase acts as a Cdc42 effector in promoting cytoskeletal reorganization. | Leung, T., et al. 1998. Mol Cell Biol. 18: 130-40. PMID: 9418861
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MRCKβ Antibody (C-12) | sc-374597 | 200 µg/ml | $322.00 | |||
MRCKβ Antibody (C-12): m-IgG Fc BP-HRP Bundle | sc-527487 | 200 µg Ab; 10 µg BP | $361.00 | |||
MRCKβ Antibody (C-12): m-IgG1 BP-HRP Bundle | sc-532860 | 200 µg Ab; 20 µg BP | $361.00 | |||
MRCKβ Antibody (C-12) AC | sc-374597 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MRCKβ Antibody (C-12) HRP | sc-374597 HRP | 200 µg/ml | $322.00 | |||
MRCKβ Antibody (C-12) FITC | sc-374597 FITC | 200 µg/ml | $336.00 | |||
MRCKβ Antibody (C-12) PE | sc-374597 PE | 200 µg/ml | $349.00 | |||
MRCKβ Antibody (C-12) Alexa Fluor® 488 | sc-374597 AF488 | 200 µg/ml | $364.00 | |||
MRCKβ Antibody (C-12) Alexa Fluor® 546 | sc-374597 AF546 | 200 µg/ml | $364.00 | |||
MRCKβ Antibody (C-12) Alexa Fluor® 594 | sc-374597 AF594 | 200 µg/ml | $364.00 | |||
MRCKβ Antibody (C-12) Alexa Fluor® 647 | sc-374597 AF647 | 200 µg/ml | $364.00 | |||
MRCKβ Antibody (C-12) Alexa Fluor® 680 | sc-374597 AF680 | 200 µg/ml | $364.00 | |||
MRCKβ Antibody (C-12) Alexa Fluor® 790 | sc-374597 AF790 | 200 µg/ml | $364.00 |