Mpk1 Antibody (E-8): sc-374440

Mpk1 Antibody (E-8) is a mouse monoclonal IgG2b antibody that detects Mpk1 in yeast by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Mpk1, also known as Slt2, is a crucial mitogen-activated protein kinase (MAPK) that plays a vital role in the cell wall integrity signaling pathway in yeast. Mpk1 is primarily located in the cytoplasm and is activated in response to cell wall stress, which is essential for maintaining cell shape and preventing lysis. Mpk1 activation is critical for yeast′s ability to adapt to environmental changes and stressors, ensuring survival and proper growth. Mutations in Mpk1 can lead to severe defects in cell wall synthesis, resulting in compromised cell integrity and increased susceptibility to external stresses. Anti-Mpk1 antibody (E-8) is an invaluable tool for researchers studying MAPK signaling pathways and their implications in cellular responses to stress, providing insights into fundamental processes that govern yeast biology and potentially informing broader applications in other eukaryotic systems.

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Mpk1 Antibody (E-8) References:

1. Regulation of the yeast Rlm1 transcription factor by the Mpk1 cell wall integrity MAP kinase.  |  Jung, US., et al. 2002. Mol Microbiol. 46: 781-9. PMID: 12410835
2. The Cryptococcus neoformans MAP kinase Mpk1 regulates cell integrity in response to antifungal drugs and loss of calcineurin function.  |  Kraus, PR., et al. 2003. Mol Microbiol. 48: 1377-87. PMID: 12787363
3. Slt2 (Mpk1) MAP kinase is involved in the response of Saccharomyces cerevisiae to 8-methoxypsoralen plus UVA.  |  Dardalhon, M., et al. 2009. J Photochem Photobiol B. 95: 148-55. PMID: 19318276
4. Transcriptional reporters for genes activated by cell wall stress through a non-catalytic mechanism involving Mpk1 and SBF.  |  Kim, KY. and Levin, DE. 2010. Yeast. 27: 541-8. PMID: 20641022
5. MPK1/SLT2 Links Multiple Stress Responses with Gene Expression in Budding Yeast by Phosphorylating Tyr1 of the RNAP II CTD.  |  Yurko, N., et al. 2017. Mol Cell. 68: 913-925.e3. PMID: 29220656
6. Functional analysis of Mpk1-mediated cell wall integrity signaling pathway in the thermotolerant methylotrophic yeast Hansenula polymorpha.  |  Kim, H., et al. 2018. J Microbiol. 56: 72-82. PMID: 29299840
7. Yeast RLM1 encodes a serum response factor-like protein that may function downstream of the Mpk1 (Slt2) mitogen-activated protein kinase pathway.  |  Watanabe, Y., et al. 1995. Mol Cell Biol. 15: 5740-9. PMID: 7565726
8. The SLT2(MPK1) MAP kinase is activated during periods of polarized cell growth in yeast.  |  Zarzov, P., et al. 1996. EMBO J. 15: 83-91. PMID: 8598209
9. Molecular and functional characterization of a mutant allele of the mitogen-activated protein-kinase gene SLT2(MPK1) rescued from yeast autolytic mutants.  |  Martín, H., et al. 1996. Curr Genet. 29: 516-22. PMID: 8662190
10. Yeast Crv4/Ttp1, a predicted type II membrane protein, is involved in an event important for growth, functionally overlapping with the event regulated by calcineurin- and Mpk1-mediated pathways.  |  Nakamura, T., et al. 1997. Mol Gen Genet. 256: 481-7. PMID: 9413431

The preferred Mpk1 antibody is Mpk1 Antibody (D-1): sc-374434.