Monomethyl Histone H4 Antibody (5E10-D8) recommended for detection of Lys 20 monomethylated Histone H4 of mouse, rat and human origin by WB and ELISA

Monomethyl Histone H4 Antibody (5E10-D8): sc-134221

Monomethyl Histone H4 Antibody (5E10-D8) is rated 4.8 out of 5 by 4.
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Datasheets

    • Monomethyl Histone H4 Antibody (5E10-D8) is a mouse monoclonal IgG1 κ, cited in 5 publications, provided at 200 µg/ml
    • raised against a short amino acid sequence containing monomethylated lysine 20 of Lys 20 monomethylated Histone H4 of human origin
    • Anti-Monomethyl Histone H4 Antibody (5E10-D8) is recommended for detection of Lys 20 monomethylated Histone H4 of mouse, rat and human origin by WB and ELISA; non cross-reactive with Histone H4 unmethylated or dimethylated at lysine 20, nor with Histone H3
    • Anti-Monomethyl Histone H4 Antibody (5E10-D8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Monomethyl Histone H4 (5E10-D8): sc-134221.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Monomethyl Histone H4 Antibody (5E10-D8) for WB applications. This reagent is now offered in a bundle with Monomethyl Histone H4 Antibody (5E10-D8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

I need to use an antibody to detect the protein in samples of monkey origin. Do you know if sc-134221: Monomethyl Histone H4 (5E10-D8) mouse monoclonal antibody will work?

Asked by: DefinitelyNotMatt
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 5 out of 5 by from This antibody is good for western blotting. I tested this antibody with preimplantaition embryos. It performs well in western blot analysis.
Date published: 2019-04-13
Rated 4 out of 5 by from Works well for yeast I tested this antibody with the yeast protein extracts. It is sensitive and specific. I would recommend using this antibody in yeast.
Date published: 2017-10-27
Rated 5 out of 5 by from Publishable immunostaining in paraffin embedded Publishable immunostaining in paraffin embedded mouse testes -SCBT Publication Review
Date published: 2015-04-08
Rated 5 out of 5 by from Clean blot with single strong band at expected Clean blot with single strong band at expected size using HeLa whole cell lysate. -SCBT QC
Date published: 2013-10-10
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