Monomethyl Histone H4 Antibody (5E10-D8) is a mouse monoclonal IgG1 κ, cited in 6 publications, provided at 200 µg/ml
raised against a short amino acid sequence containing monomethylated lysine 20 of Lys 20 monomethylated Histone H4 of human origin
Anti-Monomethyl Histone H4 Antibody (5E10-D8) is recommended for detection of Lys 20 monomethylated Histone H4 of mouse, rat and human origin by WB and ELISA; non cross-reactive with Histone H4 unmethylated or dimethylated at lysine 20, nor with Histone H3
Anti-Monomethyl Histone H4 Antibody (5E10-D8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Monomethyl Histone H4 (5E10-D8): sc-134221.
m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Monomethyl Histone H4 Antibody (5E10-D8) for WB applications. These reagents are now offered in bundles with Monomethyl Histone H4 Antibody (5E10-D8) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fibers. Two molecules of each of the four core histones (H2A, H2B, H3 and H4) form the octamer, which is comprised of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Human and mouse Histone H4 are subject to methylation at Lys 20, a modification that may be necessary for select DNA transactions or chromatin state transitions.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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