MOF Antibody (G-12): sc-271691

MOF Antibody (G-12) is a mouse monoclonal IgG1 antibody that detects MOF in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MOF, also known as hMOF, MYST1, or MOZ, plays a crucial role in gene expression regulation by functioning as a histone acetyltransferase, essential for dosage compensation in organisms like Drosophila. MOF is primarily located in the nucleus, where MOF interacts with chromatin to modify histones, particularly acetylating histone H4 at lysine 16. This modification facilitates dosage compensation complex recruitment, enhancing transcription levels of the male X chromosome to ensure equal expression of X-linked genes between males and females. MOF′s unique structure, characterized by a C2HC-type zinc finger near its histone acetyltransferase domain, allows effective binding to nucleosomes and histone tails, critical for enzymatic activity and overall function in gene regulation. Through interactions with other proteins in the MYST family, MOF is integral to maintaining proper gene expression and cellular function, making anti-MOF antibody (G-12) an essential tool for researchers studying epigenetic regulation and chromatin dynamics.

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MOF Antibody (G-12) References:

1. The histone acetyltransferase hMOF is frequently downregulated in primary breast carcinoma and medulloblastoma and constitutes a biomarker for clinical outcome in medulloblastoma.  |  Pfister, S., et al. 2008. Int J Cancer. 122: 1207-13. PMID: 18058815
2. Mof (MYST1 or KAT8) is essential for progression of embryonic development past the blastocyst stage and required for normal chromatin architecture.  |  Thomas, T., et al. 2008. Mol Cell Biol. 28: 5093-105. PMID: 18541669
3. Role of hMOF-dependent histone H4 lysine 16 acetylation in the maintenance of TMS1/ASC gene activity.  |  Kapoor-Vazirani, P., et al. 2008. Cancer Res. 68: 6810-21. PMID: 18701507
4. Acetylated H4K16 by MYST1 protects UROtsa cells from arsenic toxicity and is decreased following chronic arsenic exposure.  |  Jo, WJ., et al. 2009. Toxicol Appl Pharmacol. 241: 294-302. PMID: 19732783
5. Histone acetyltransferase hMOF promotes S phase entry and tumorigenesis in lung cancer.  |  Zhao, L., et al. 2013. Cell Signal. 25: 1689-98. PMID: 23628702
6. hMOF acetylation of DBC1/CCAR2 prevents binding and inhibition of SirT1.  |  Zheng, H., et al. 2013. Mol Cell Biol. 33: 4960-70. PMID: 24126058
7. Coactivator MYST1 regulates nuclear factor-κB and androgen receptor functions during proliferation of prostate cancer cells.  |  Jaganathan, A., et al. 2014. Mol Endocrinol. 28: 872-85. PMID: 24702180
8. Expression of hMOF in different ovarian tissues and its effects on ovarian cancer prognosis.  |  Cai, M., et al. 2015. Oncol Rep. 33: 685-92. PMID: 25483274
9. hMOF reduction enhances radiosensitivity through the homologous recombination pathway in non-small-cell lung cancer.  |  Li, N., et al. 2019. Onco Targets Ther. 12: 3065-3075. PMID: 31114249
10. MYST1/KAT8 contributes to tumor progression by activating EGFR signaling in glioblastoma cells.  |  Dong, Z., et al. 2019. Cancer Med. 8: 7793-7808. PMID: 31691527

The preferred MOF antibody is MOF Antibody (8C4C4): sc-81163.