MOF Antibody (8C4C4): sc-81163
- MOF Antibody (8C4C4) is a mouse monoclonal IgG1 κ MOF antibody, cited in 1 publications, provided at 200 µg/ml
- raised against a recombinant protein corresponding to amino acids 132-283 of MOF of human origin
- MOF Antibody (8C4C4) is recommended for detection of MOF of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-MOF Antibody (8C4C4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for MOF Antibody (8C4C4) for WB and IHC(P) applications. This reagent is now offered in a bundle with MOF Antibody (8C4C4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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MOF Antibody (8C4C4) is a mouse monoclonal IgG1 antibody that detects the MOF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-MOF antibody (8C4C4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The MOF protein plays a crucial role in dosage compensation, a process that ensures equal expression of X-linked genes in males and females. In Drosophila, this process is achieved through the formation of a dosage compensation complex (DCC) that includes proteins such as maleless and male-specific lethal proteins, which work together to enhance transcription from the male X chromosome. MOF is essential for this process as MOF acetylates histone H4 at lysine 16, a modification that facilitates the binding of the DCC to the X chromosome. In mammals, MOF, also known as hMOF, MYST1, or MOZ, is part of the MYST family of histone acetyltransferases, characterized by a unique C2HC-type zinc finger that interacts with nucleosomes and histone substrates. This structural feature is vital for MOF′s enzymatic activity, allowing MOF to regulate gene expression through histone modification, thereby influencing various biological processes, including development and cellular response to environmental stimuli.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MOF Antibody (8C4C4) References:
- A new human member of the MYST family of histone acetyl transferases with high sequence similarity to Drosophila MOF. | Neal, KC., et al. 2000. Biochim Biophys Acta. 1490: 170-4. PMID: 10786633
- Acetylation of histones and transcription-related factors. | Sterner, DE. and Berger, SL. 2000. Microbiol Mol Biol Rev. 64: 435-59. PMID: 10839822
- Activation of transcription through histone H4 acetylation by MOF, an acetyltransferase essential for dosage compensation in Drosophila. | Akhtar, A. and Becker, PB. 2000. Mol Cell. 5: 367-75. PMID: 10882077
- Chromodomains are protein-RNA interaction modules. | Akhtar, A., et al. 2000. Nature. 407: 405-9. PMID: 11014199
- The histone H4 acetyltransferase MOF uses a C2HC zinc finger for substrate recognition. | Akhtar, A. and Becker, PB. 2001. EMBO Rep. 2: 113-8. PMID: 11258702
- hMOF histone acetyltransferase is required for histone H4 lysine 16 acetylation in mammalian cells. | Taipale, M., et al. 2005. Mol Cell Biol. 25: 6798-810. PMID: 16024812
- Structure and function of MYST1 histone acetyltransferase in the interactome of animal cells. | Dmitriev, RI., et al. 2008. Biochemistry (Mosc). 73: 839-52. PMID: 18774930
- The histone H4 lysine 16 acetyltransferase hMOF regulates the outcome of autophagy. | Füllgrabe, J., et al. 2013. Nature. 500: 468-71. PMID: 23863932
- Lysine acetyltransferase 8 is involved in cerebral development and syndromic intellectual disability. | Li, L., et al. 2020. J Clin Invest. 130: 1431-1445. PMID: 31794431
- KAT8, lysine acetyltransferase 8, is required for adipocyte differentiation in vitro. | Burrell, JA. and Stephens, JM. 2021. Biochim Biophys Acta Mol Basis Dis. 1867: 166103. PMID: 33617987
- mof, a putative acetyl transferase gene related to the Tip60 and MOZ human genes and to the SAS genes of yeast, is required for dosage compensation in Drosophila. | Hilfiker, A., et al. 1997. EMBO J. 16: 2054-60. PMID: 9155031
- Targeting of MOF, a putative histone acetyl transferase, to the X chromosome of Drosophila melanogaster. | Gu, W., et al. 1998. Dev Genet. 22: 56-64. PMID: 9499580
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MOF Antibody (8C4C4) | sc-81163 | 200 µg/ml | $322.00 | |||
MOF Antibody (8C4C4): m-IgGκ BP-HRP Bundle | sc-521167 | 200 µg Ab, 40 µg BP | $361.00 | |||
MOF Antibody (8C4C4) AC | sc-81163 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MOF Antibody (8C4C4) HRP | sc-81163 HRP | 200 µg/ml | $322.00 | |||
MOF Antibody (8C4C4) FITC | sc-81163 FITC | 200 µg/ml | $336.00 | |||
MOF Antibody (8C4C4) PE | sc-81163 PE | 200 µg/ml | $349.00 | |||
MOF Antibody (8C4C4) Alexa Fluor® 488 | sc-81163 AF488 | 200 µg/ml | $364.00 | |||
MOF Antibody (8C4C4) Alexa Fluor® 546 | sc-81163 AF546 | 200 µg/ml | $364.00 | |||
MOF Antibody (8C4C4) Alexa Fluor® 594 | sc-81163 AF594 | 200 µg/ml | $364.00 | |||
MOF Antibody (8C4C4) Alexa Fluor® 647 | sc-81163 AF647 | 200 µg/ml | $364.00 | |||
MOF Antibody (8C4C4) Alexa Fluor® 680 | sc-81163 AF680 | 200 µg/ml | $364.00 | |||
MOF Antibody (8C4C4) Alexa Fluor® 790 | sc-81163 AF790 | 200 µg/ml | $364.00 |