MOB1A Antibody (G-7): sc-393212
- MOB1A Antibody (G-7) is a mouse monoclonal IgG1 κ MOB1A antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1-64 mapping at the N-terminus of MOB1A of human origin
- MOB1A Antibody (G-7) is recommended for detection of MOB1A of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine and bovine
- Anti-MOB1A Antibody (G-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MOB1A Antibody (G-7) for WB applications. These reagents are now offered in bundles with MOB1A Antibody (G-7) (see ordering information below).
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MOB1A Antibody (G-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects MOB1A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-MOB1A antibody (G-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MOB1A protein plays a crucial role in cell cycle regulation and tumor suppression, functioning as a key component of the Hippo signaling pathway, which is essential for controlling cell growth and preventing excessive cell proliferation. This pathway is particularly important in maintaining tissue homeostasis and preventing cancer development, as MOB1A regulates the activity of downstream effectors like NDR-family protein kinases and LATS1 kinase. Through binding and modulating these kinases, MOB1A helps orchestrate cellular responses to various stimuli, including stress and growth factors, thereby influencing cell fate decisions. MOB1A′s interaction with these critical regulators underscores MOB1A′s significance in cellular signaling and potential as a therapeutic target in cancer treatment.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MOB1A Antibody (G-7) References:
- The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein beta gamma subunits to downstream signalling components. | Leberer, E., et al. 1992. EMBO J. 11: 4815-24. PMID: 1464311
- Mechanism of activation of NDR (nuclear Dbf2-related) protein kinase by the hMOB1 protein. | Bichsel, SJ., et al. 2004. J Biol Chem. 279: 35228-35. PMID: 15197186
- Human LATS1 is a mitotic exit network kinase. | Bothos, J., et al. 2005. Cancer Res. 65: 6568-75. PMID: 16061636
- Human NDR kinases are rapidly activated by MOB proteins through recruitment to the plasma membrane and phosphorylation. | Hergovich, A., et al. 2005. Mol Cell Biol. 25: 8259-72. PMID: 16135814
- The human tumour suppressor LATS1 is activated by human MOB1 at the membrane. | Hergovich, A., et al. 2006. Biochem Biophys Res Commun. 345: 50-8. PMID: 16674920
- Human MOB1 expression in non-small-cell lung cancer. | Sasaki, H., et al. 2007. Clin Lung Cancer. 8: 273-6. PMID: 17311693
- MOBKL1A/MOBKL1B phosphorylation by MST1 and MST2 inhibits cell proliferation. | Praskova, M., et al. 2008. Curr Biol. 18: 311-21. PMID: 18328708
- The Hippo pathway in intestinal regeneration and disease. | Hong, AW., et al. 2016. Nat Rev Gastroenterol Hepatol. 13: 324-37. PMID: 27147489
- Loss of Mob1a/b impairs the differentiation of mouse embryonic stem cells into the three germ layer lineages. | Bae, JS., et al. 2019. Exp Mol Med. 51: 1-12. PMID: 31723125
- MOB1A regulates glucose deprivation-induced autophagy via IL6-STAT3 pathway in gallbladder carcinoma. | Yang, B., et al. 2020. Am J Cancer Res. 10: 3896-3910. PMID: 33294275
- CAF-derived exosomal WEE2-AS1 facilitates colorectal cancer progression via promoting degradation of MOB1A to inhibit the Hippo pathway. | Yang, P., et al. 2022. Cell Death Dis. 13: 796. PMID: 36123327
- Cloning and characterization of a human STE20-like protein kinase with unusual cofactor requirements. | Schinkmann, K. and Blenis, J. 1997. J Biol Chem. 272: 28695-703. PMID: 9353338
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MOB1A Antibody (G-7) | sc-393212 | 200 µg/ml | $322.00 | |||
MOB1A Antibody (G-7): m-IgG Fc BP-HRP Bundle | sc-530393 | 200 µg Ab; 10 µg BP | $361.00 | |||
MOB1A Antibody (G-7): m-IgGκ BP-HRP Bundle | sc-523856 | 200 µg Ab, 40 µg BP | $361.00 | |||
MOB1A Antibody (G-7) AC | sc-393212 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MOB1A Antibody (G-7) HRP | sc-393212 HRP | 200 µg/ml | $322.00 | |||
MOB1A Antibody (G-7) FITC | sc-393212 FITC | 200 µg/ml | $336.00 | |||
MOB1A Antibody (G-7) PE | sc-393212 PE | 200 µg/ml | $349.00 | |||
MOB1A Antibody (G-7) Alexa Fluor® 488 | sc-393212 AF488 | 200 µg/ml | $364.00 | |||
MOB1A Antibody (G-7) Alexa Fluor® 546 | sc-393212 AF546 | 200 µg/ml | $364.00 | |||
MOB1A Antibody (G-7) Alexa Fluor® 594 | sc-393212 AF594 | 200 µg/ml | $364.00 | |||
MOB1A Antibody (G-7) Alexa Fluor® 647 | sc-393212 AF647 | 200 µg/ml | $364.00 | |||
MOB1A Antibody (G-7) Alexa Fluor® 680 | sc-393212 AF680 | 200 µg/ml | $364.00 | |||
MOB1A Antibody (G-7) Alexa Fluor® 790 | sc-393212 AF790 | 200 µg/ml | $364.00 |