MND1 Antibody (G-4): sc-377319

MND1 Antibody (G-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects MND1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MND1 (G-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MND1, or meiotic nuclear division protein 1 homolog, is a crucial 205 amino acid nuclear protein that plays a vital role in homologous chromosome pairing and meiotic double-strand break repair, which are essential processes for accurate genetic recombination during meiosis. MND1 protein is primarily localized to chromatin during meiotic prophase, where MND1 preferentially binds to double-stranded DNA, facilitating the formation of a stable heterodimeric complex with HOP2. This complex is critical for activating the recombinase activity of DMC1 and RAD51, both of which are necessary for effective meiotic recombination. Disruption of the MND1-HOP2 complex can lead to severe defects in homologous chromosome synapsis and meiotic recombination, underscoring MND1′s importance in maintaining genomic stability. The MND1 gene is located on human chromosome 4, a region that is significant not only for its size, housing nearly 6% of the human genome, but also for containing some of the largest gene deserts in the human genome. Notably, defects in genes on chromosome 4 have been linked to various genetic disorders, including Huntington′s disease, Ellis-van Creveld syndrome, methylmalonic acidemia, and polycystic kidney disease, highlighting the broader implications of MND1′s function in cellular processes.

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MND1 Antibody (G-4) References:

1. The Mnd1 protein forms a complex with hop2 to promote homologous chromosome pairing and meiotic double-strand break repair.  |  Tsubouchi, H. and Roeder, GS. 2002. Mol Cell Biol. 22: 3078-88. PMID: 11940665
2. Identification of the gene responsible for the cblA complementation group of vitamin B12-responsive methylmalonic acidemia based on analysis of prokaryotic gene arrangements.  |  Dobson, CM., et al. 2002. Proc Natl Acad Sci U S A. 99: 15554-9. PMID: 12438653
3. Polycystic kidneys and del (4)(q21.1q21.3): further delineation of a distinct phenotype.  |  Velinov, M., et al. 2005. Eur J Med Genet. 48: 51-5. PMID: 15953406
4. Stimulation of DNA strand exchange by the human TBPIP/Hop2-Mnd1 complex.  |  Enomoto, R., et al. 2006. J Biol Chem. 281: 5575-81. PMID: 16407260
5. Bipartite stimulatory action of the Hop2-Mnd1 complex on the Rad51 recombinase.  |  Chi, P., et al. 2007. Genes Dev. 21: 1747-57. PMID: 17639080
6. Hop2/Mnd1 acts on two critical steps in Dmc1-promoted homologous pairing.  |  Pezza, RJ., et al. 2007. Genes Dev. 21: 1758-66. PMID: 17639081
7. A Turkish family with Ellis-van Creveld syndrome in six siblings; linkage analysis on 4p16 region (D4S3360-D4S2366).  |  Cağdaş, DN., et al. 2008. Genet Couns. 19: 387-95. PMID: 19239082
8. 4p16.3 haplotype modifying age at onset of Huntington disease.  |  Nørremølle, A., et al. 2009. Clin Genet. 75: 244-50. PMID: 19250382
9. MND1 and PSMC3IP control PARP inhibitor sensitivity in mitotic cells.  |  Zelceski, A., et al. 2023. Cell Rep. 42: 112484. PMID: 37163373
10. The Hop2-Mnd1 Complex and Its Regulation of Homologous Recombination.  |  Tsubouchi, H. 2023. Biomolecules. 13: PMID: 37189409
11. Hop2-Mnd1 and Swi5-Sfr1 stimulate Dmc1 filament assembly using distinct mechanisms.  |  Lee, W., et al. 2023. Nucleic Acids Res. 51: 8550-8562. PMID: 37395447
12. Hop2-Mnd1 functions as a DNA sequence fidelity switch in Dmc1-mediated DNA recombination.  |  Peng, JC., et al. 2024. Nat Commun. 15: 9266. PMID: 39463417

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MND1 Antibody (G-4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-MND1 Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MND1 Antibody (G-4) Specifications

Alternate Product Name: MND1

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 181-203

Epitope Target: MND1

Epitope Species: human

Product Reactivity/Detection: MND1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for MND1 Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent