MMP-21 Antibody (C-7): sc-398935

MMP-21 Antibody (C-7) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect human matrix metalloproteinase-21 (MMP-21) with precision in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MMP-21 monoclonal antibody (C-7) targets an epitope mapping between amino acids 241-263 within an internal region of MMP-21, ensuring robust reactivity with MMP-21 from mouse, rat, and human origins. MMP-21, also called HTX7 and MMP21, is a 569 amino acid enzyme that plays a crucial role in the degradation of extracellular matrix (ECM) components, including collagen, gelatin, fibronectin, laminin, and proteoglycans, which are essential for processes such as embryogenesis, tissue development, tumor progression, and apoptosis. Structurally, MMP-21 consists of a prodomain, catalytic domain, and hemopexin-like domain, and undergoes significant post-translational modifications vital for activation and function. MMP-21 is synthesized as an inactive proenzyme and requires cleavage of its prodomain by extracellular proteinases to become enzymatically active, a modification essential for ECM remodeling and cellular migration. Additionally, glycosylation of MMP-21 enhances stability, localization, and interactions with other proteins, such as tissue inhibitors of metalloproteinases (TIMPs) and integrins, which regulate proteolytic activity and facilitate involvement in cell adhesion and invasion. The precise regulation of these post-translational modifications is critical for maintaining the balance between ECM degradation and tissue integrity, and dysregulation can contribute to pathological conditions like cancer metastasis and abnormal tissue development. Anti-MMP-21 antibody (C-7) enables researchers to investigate the intricate mechanisms of MMP-21 regulation, interactions with other proteins, and impact on various physiological and pathological processes, making this tool indispensable for advancing biomedical research and developing targeted therapeutic strategies.

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MMP-21 Antibody (C-7) References:

1. Matrix metalloproteinase-21, the human orthologue for XMMP, is expressed during fetal development and in cancer.  |  Ahokas, K., et al. 2002. Gene. 301: 31-41. PMID: 12490321
2. The structure and regulation of the human and mouse matrix metalloproteinase-21 gene and protein.  |  Marchenko, GN., et al. 2003. Biochem J. 372: 503-15. PMID: 12617721
3. The matrix metalloproteinase-21 gene 572C/T polymorphism and the risk of breast cancer.  |  Shagisultanova, EI., et al. 2004. Anticancer Res. 24: 199-201. PMID: 15015597
4. MMP-21 is upregulated at early stages of melanoma progression but disappears with more aggressive phenotype.  |  Kuivanen, T., et al. 2005. Virchows Arch. 447: 954-60. PMID: 16133364
5. Matrix metalloproteinases 21 and 26 are differentially expressed in esophageal squamous cell cancer.  |  Ahokas, K., et al. 2006. Tumour Biol. 27: 133-41. PMID: 16641547
6. MMP-21 is expressed by macrophages and fibroblasts in vivo and in culture.  |  Skoog, T., et al. 2006. Exp Dermatol. 15: 775-83. PMID: 16984259
7. Structural implications for the role of the N terminus in the 'superactivation' of collagenases. A crystallographic study.  |  Reinemer, P., et al. 1994. FEBS Lett. 338: 227-33. PMID: 8307185
8. Matrix metalloproteinases: a review.  |  Birkedal-Hansen, H., et al. 1993. Crit Rev Oral Biol Med. 4: 197-250. PMID: 8435466
9. Expression of several matrix metalloproteinase genes in human monocytic cells.  |  Machein, U. and Conca, W. 1997. Adv Exp Med Biol. 421: 247-51. PMID: 9330704

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MMP-21 Antibody (C-7) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-MMP-21 Antibody (C-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MMP-21 Antibody (C-7) Specifications

Alternate Product Name: MMP-21

Product Clone Name: C-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 241-263

Epitope Target: MMP-21

Epitope Species: human

Product Reactivity/Detection: MMP-21

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for MMP-21 Antibody (C-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin