MMP-12 Antibody (G-2) recommended for detection of MMP-12 of mouse, rat and human origin by WB, IP, IF and ELISA

MMP-12 Antibody (G-2): sc-390863

MMP-12 Antibody (G-2) is rated 5.0 out of 5 by 1.
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Datasheets

    • MMP-12 Antibody (G-2) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 438-453 near the C-terminus of MMP-12 of mouse origin
    • Anti-MMP-12 Antibody (G-2) is recommended for detection of MMP-12 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-MMP-12 Antibody (G-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-390863 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MMP-12 (G-2): sc-390863.

Does this antibody target the pro-form or active form of Mmp12 or both?

Asked by: Zhipeng
Thank you for your question. The zymogen form for MMP-12 is about ~54 kDa, which is activated to the 45-48 kDa form, which further breaks down to a cascade of active forms and resulting in a common 22 kDa form. Which form you will see depends on what form is present in your sample. Our data with this antibody generally shows the active 48kDa form.
Answered by: Tech Service
Date published: 2019-10-08

Dear Madam/Sir, I am write in asking for how to receive FREE 10 µg sample of MMP-12 (G-2): sc-390863. I want to give a try before buying some for my research

Asked by: YuanCao
Thank you for your question. Please contact one of our authorized distributors in Australia to request a free 10 µg sample of this monoclonal antibody. Please use the link below to search for their contact information on our website. https://www.scbt.com/scbt/resources/distributors
Answered by: Technical Support
Date published: 2017-10-12

What application is the blocking peptide sc-390863 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Specific and strong band detected by Western Specific and strong band detected by Western blot analysis of MMP-12 expression in AMJ2-C8 whole cell lysate. - SCBT QC
Date published: 2015-03-06
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