MLL5 Antibody (C-10): sc-377182
- MLL5 Antibody (C-10) is a mouse monoclonal IgG1 κ MLL5 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 81-190 mapping near the N-terminus of MLL5 of human origin
- MLL5 Antibody (C-10) is recommended for detection of MLL5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine and porcine
- Anti-MLL5 Antibody (C-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-377182 X, 200 µg/0.1 ml)
- m-IgGκ BP-HRP is the preferred secondary detection reagent for MLL5 Antibody (C-10) for WB and IHC(P) applications. This reagent is now offered in a bundle with MLL5 Antibody (C-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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MLL5 Antibody (C-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects MLL5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). MLL5 Antibody (C-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MLL5 protein is a member of the mixed lineage leukemia (MLL) gene family, which consists of histone H3 lysine 4 (H3K4) methyltransferases that play crucial roles in gene regulation and chromatin remodeling. MLL5 is particularly important for maintaining proper cellular growth and differentiation, as MLL5 is involved in the formation of intranuclear protein complexes that regulate chromatin structure. This regulation is vital for controlling gene expression patterns during development and in response to cellular signals. The gene encoding human MLL5 is located on chromosome band 7q22, a region frequently associated with cytogenetic abnormalities in acute myeloid malignancies, highlighting MLL5′s potential role in cancer biology. MLL5′s localization to the nucleus underscores MLL5′s function in modulating chromatin dynamics, which is essential for the suppression of uncontrolled cellular proliferation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MLL5 Antibody (C-10) References:
- MLL2, the second human homolog of the Drosophila trithorax gene, maps to 19q13.1 and is amplified in solid tumor cell lines. | Huntsman, DG., et al. 1999. Oncogene. 18: 7975-84. PMID: 10637508
- MLL3, a new human member of the TRX/MLL gene family, maps to 7q36, a chromosome region frequently deleted in myeloid leukaemia. | Ruault, M., et al. 2002. Gene. 284: 73-81. PMID: 11891048
- MLL 5 protein forms intranuclear foci, and overexpression inhibits cell cycle progression. | Deng, LW., et al. 2004. Proc Natl Acad Sci U S A. 101: 757-62. PMID: 14718661
- Coactivator as a target gene specificity determinant for histone H3 lysine 4 methyltransferases. | Lee, S., et al. 2006. Proc Natl Acad Sci U S A. 103: 15392-7. PMID: 17021013
- Cross-talk between histone modifications in response to histone deacetylase inhibitors: MLL4 links histone H3 acetylation and histone H3K4 methylation. | Nightingale, KP., et al. 2007. J Biol Chem. 282: 4408-4416. PMID: 17166833
- Knockdown of ALR (MLL2) reveals ALR target genes and leads to alterations in cell adhesion and growth. | Issaeva, I., et al. 2007. Mol Cell Biol. 27: 1889-903. PMID: 17178841
- Increased apoptosis and skewed differentiation in mouse embryonic stem cells lacking the histone methyltransferase Mll2. | Lubitz, S., et al. 2007. Mol Biol Cell. 18: 2356-66. PMID: 17429066
- Activating signal cointegrator-2 is an essential adaptor to recruit histone H3 lysine 4 methyltransferases MLL3 and MLL4 to the liver X receptors. | Lee, S., et al. 2008. Mol Endocrinol. 22: 1312-9. PMID: 18372346
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MLL5 Antibody (C-10) | sc-377182 | 200 µg/ml | $322.00 | |||
MLL5 Antibody (C-10): m-IgGκ BP-HRP Bundle | sc-551104 | 200 µg Ab; 40 µg BP | $361.00 | |||
MLL5 Antibody (C-10) X | sc-377182 X | 200 µg/0.1 ml | $322.00 | |||
MLL5 Antibody (C-10) AC | sc-377182 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MLL5 Antibody (C-10) HRP | sc-377182 HRP | 200 µg/ml | $322.00 | |||
MLL5 Antibody (C-10) FITC | sc-377182 FITC | 200 µg/ml | $336.00 | |||
MLL5 Antibody (C-10) PE | sc-377182 PE | 200 µg/ml | $349.00 | |||
MLL5 Antibody (C-10) Alexa Fluor® 488 | sc-377182 AF488 | 200 µg/ml | $364.00 | |||
MLL5 Antibody (C-10) Alexa Fluor® 546 | sc-377182 AF546 | 200 µg/ml | $364.00 | |||
MLL5 Antibody (C-10) Alexa Fluor® 594 | sc-377182 AF594 | 200 µg/ml | $364.00 | |||
MLL5 Antibody (C-10) Alexa Fluor® 647 | sc-377182 AF647 | 200 µg/ml | $364.00 | |||
MLL5 Antibody (C-10) Alexa Fluor® 680 | sc-377182 AF680 | 200 µg/ml | $364.00 | |||
MLL5 Antibody (C-10) Alexa Fluor® 790 | sc-377182 AF790 | 200 µg/ml | $364.00 |