MLKL Antibody (3B2): sc-293201

MLKL Antibody (3B2) is a mouse monoclonal IgG2a kappa light chain antibody that detects MLKL protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-MLKL antibody (3B2) is available as a non-conjugated format. Mixed lineage kinase domain-like protein (MLKL) is a crucial component in necroptosis, a form of programmed cell death that plays a significant role in inflammation and tissue homeostasis. This 471 amino acid protein features a protein kinase domain that is believed to be catalytically inactive, yet remains essential for recruiting other signaling molecules during necroptosis. MLKL is encoded on chromosome 16, which is associated with various genetic disorders, including Crohn′s disease and Rubinstein-Taybi syndrome. Understanding MLKL function is vital, as dysregulation can lead to pathological conditions, including neurodegenerative diseases and inflammatory disorders. Anti-MLKL antibody (3B2) enables researchers to gain insights into necroptosis mechanisms and their implications in human health and disease.

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MLKL Antibody (3B2) References:

1. Disease genes and chromosomes: disease maps of the human genome. Chromosome 16.  |  Gilbert, F. 1999. Genet Test. 3: 243-54. PMID: 10464676
2. The protein kinase complement of the human genome.  |  Manning, G., et al. 2002. Science. 298: 1912-34. PMID: 12471243
3. Analysis of CBP (CREBBP) gene deletions in Rubinstein-Taybi syndrome patients using real-time quantitative PCR.  |  Coupry, I., et al. 2004. Hum Mutat. 23: 278-84. PMID: 14974086
4. The sequence and analysis of duplication-rich human chromosome 16.  |  Martin, J., et al. 2004. Nature. 432: 988-94. PMID: 15616553
5. Giant axonal neuropathy: clinical and genetic study in six cases.  |  Demir, E., et al. 2005. J Neurol Neurosurg Psychiatry. 76: 825-32. PMID: 15897506
6. Chromosome 16 tumor-suppressor genes in breast cancer.  |  Rakha, EA., et al. 2006. Genes Chromosomes Cancer. 45: 527-35. PMID: 16518845
7. High frequency of mosaic CREBBP deletions in Rubinstein-Taybi syndrome patients and mapping of somatic and germ-line breakpoints.  |  Gervasini, C., et al. 2007. Genomics. 90: 567-73. PMID: 17855048
8. CAMK2/CaMKII activates MLKL in short-term starvation to facilitate autophagic flux.  |  Zhan, Q., et al. 2022. Autophagy. 18: 726-744. PMID: 34282994
9. ZBP1-MLKL necroptotic signaling potentiates radiation-induced antitumor immunity via intratumoral STING pathway activation.  |  Yang, Y., et al. 2021. Sci Adv. 7: eabf6290. PMID: 34613770
10. RIPK3-MLKL necroptotic signalling amplifies STING pathway and exacerbates lethal sepsis.  |  Zhang, X., et al. 2023. Clin Transl Med. 13: e1334. PMID: 37475188
11. MLKL polymerization-induced lysosomal membrane permeabilization promotes necroptosis.  |  Liu, S., et al. 2024. Cell Death Differ. 31: 40-52. PMID: 37996483

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MLKL Antibody (3B2) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-MLKL Antibody (3B2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MLKL Antibody (3B2) Specifications

Alternate Product Name: Mixed Lineage Kinase Domain-Like

Product Clone Name: 3B2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 371-471

Epitope Target: MLKL

Epitope Species: human

Product Reactivity/Detection: MLKL

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for MLKL Antibody (3B2): N/A