MLK2 Antibody (H-4): sc-393675

MLK2 Antibody (H-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects MLK2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MLK2 (H-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MLK2, a member of the mixed lineage kinase family, plays a crucial role in cellular signaling pathways, particularly in apoptosis and stress response. MLK2 is primarily located in the cytoplasm and mediates interactions between small GTPases such as Rac1 and Cdc42, and mitogen-activated protein kinase (MAPK) pathways, which are essential for regulating cell survival and death. MLK2′s cytoplasmic localization enables rapid response to cellular stress signals, facilitating the activation of downstream effectors that can lead to either cell survival or apoptosis. MLK2 contains a Src homology 3 (SH3) domain, which is critical for protein-protein interactions, further underscoring MLK2′s role in complex signaling networks. Understanding MLK2′s function and localization is vital for elucidating contributions to various physiological and pathological processes, including cancer progression and neurodegenerative diseases.

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MLK2 Antibody (H-4) References:

1. Activation of MLK2-mediated signaling cascades by polyglutamine-expanded huntingtin.  |  Liu, YF., et al. 2000. J Biol Chem. 275: 19035-40. PMID: 10801775
2. The MLK family mediates c-Jun N-terminal kinase activation in neuronal apoptosis.  |  Xu, Z., et al. 2001. Mol Cell Biol. 21: 4713-24. PMID: 11416147
3. PAK interacts with NCK and MLK2 to regulate the activation of jun N-terminal kinase.  |  Poitras, L., et al. 2003. FEBS Lett. 543: 129-35. PMID: 12753919
4. Stimulation of NeuroD activity by huntingtin and huntingtin-associated proteins HAP1 and MLK2.  |  Marcora, E., et al. 2003. Proc Natl Acad Sci U S A. 100: 9578-83. PMID: 12881483
5. Mice lacking both mixed-lineage kinase genes Mlk1 and Mlk2 retain a wild type phenotype.  |  Bisson, N., et al. 2008. Cell Cycle. 7: 909-16. PMID: 18414056
6. MLK-3: identification of a widely-expressed protein kinase bearing an SH3 domain and a leucine zipper-basic region domain.  |  Ing, YL., et al. 1994. Oncogene. 9: 1745-50. PMID: 8183572
7. Identification of a new family of human epithelial protein kinases containing two leucine/isoleucine-zipper domains.  |  Dorow, DS., et al. 1993. Eur J Biochem. 213: 701-10. PMID: 8477742
8. Complete nucleotide sequence, expression, and chromosomal localisation of human mixed-lineage kinase 2.  |  Dorow, DS., et al. 1995. Eur J Biochem. 234: 492-500. PMID: 8536694
9. Two-dimensional electrophoretic analysis of human breast carcinoma proteins: mapping of proteins that bind to the SH3 domain of mixed lineage kinase MLK2.  |  Rasmussen, RK., et al. 1997. Electrophoresis. 18: 588-98. PMID: 9150946
10. MST/MLK2, a member of the mixed lineage kinase family, directly phosphorylates and activates SEK1, an activator of c-Jun N-terminal kinase/stress-activated protein kinase.  |  Hirai, Si., et al. 1997. J Biol Chem. 272: 15167-73. PMID: 9182538
11. The MAP kinase kinase kinase MLK2 co-localizes with activated JNK along microtubules and associates with kinesin superfamily motor KIF3.  |  Nagata, Ki., et al. 1998. EMBO J. 17: 149-58. PMID: 9427749
12. Dimerization via tandem leucine zippers is essential for the activation of the mitogen-activated protein kinase kinase kinase, MLK-3.  |  Leung, IW. and Lassam, N. 1998. J Biol Chem. 273: 32408-15. PMID: 9829970

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MLK2 Antibody (H-4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-MLK2 Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MLK2 Antibody (H-4) Specifications

Alternate Product Name: MLK2

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 837-860

Epitope Target: MLK2

Epitope Species: human

Product Reactivity/Detection: MLK2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for MLK2 Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin