MKP-4 Antibody (D-3): sc-137010
- MKP-4 Antibody (D-3) is a mouse monoclonal IgG2a κ MKP-4 antibody provided at 200 µg/ml
- raised against amino acids 340-384 mapping at the C-terminus of MKP-4 of human origin
- MKP-4 Antibody (D-3) is recommended for detection of MKP-4 of mouse, rat, human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-MKP-4 Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MKP-4 Antibody (D-3) for WB and IHC(P) applications. These reagents are now offered in bundles with MKP-4 Antibody (D-3) (see ordering information below).
MKP-4 Antibody (D-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects MKP-4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). MKP-4 (D-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MKP-4, a member of the dual specificity phosphatase family, plays a crucial role in regulating mitogen-activated protein (MAP) kinase signaling pathways, which are essential for cellular responses to growth factors and stress signals. By selectively dephosphorylating critical phosphothreonine and phosphotyrosine residues within MAP kinases, MKP-4 serves as a negative regulator of the ERK family of MAP kinases, thereby influencing various physiological processes such as cell proliferation, differentiation, and apoptosis. MKP-4 expression occurs primarily in the placenta, kidney, and fetal liver, highlighting its importance in developmental and tissue-specific functions. Additionally, MKP-4 interacts with other members of the dual-specificity phosphatase family, including MKP-1 and MKP-2, which further underscores its role in modulating MAP kinase activity and maintaining cellular homeostasis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MKP-4 Antibody (D-3) References:
- Dual specificity phosphatases: a gene family for control of MAP kinase function. | Camps, M., et al. 2000. FASEB J. 14: 6-16. PMID: 10627275
- An emerging family of dual specificity MAP kinase phosphatases. | Keyse, SM. 1995. Biochim Biophys Acta. 1265: 152-60. PMID: 7696343
- Molecular cloning and functional characterization of a novel mitogen-activated protein kinase phosphatase, MKP-4. | Muda, M., et al. 1997. J Biol Chem. 272: 5141-51. PMID: 9030581
- Functional studies of dual-specificity phosphatases. | Sun, H. 1998. Methods Mol Biol. 84: 307-18. PMID: 9666459
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MKP-4 Antibody (D-3) | sc-137010 | 200 µg/ml | $322.00 | |||
MKP-4 Antibody (D-3): m-IgG Fc BP-HRP Bundle | sc-528836 | 200 µg Ab; 10 µg BP | $361.00 | |||
MKP-4 Antibody (D-3): m-IgGκ BP-HRP Bundle | sc-521392 | 200 µg Ab, 40 µg BP | $361.00 | |||
MKP-4 Antibody (D-3): m-IgG2a BP-HRP Bundle | sc-547180 | 200 µg Ab; 10 µg BP | $361.00 | |||
MKP-4 Antibody (D-3) AC | sc-137010 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MKP-4 Antibody (D-3) HRP | sc-137010 HRP | 200 µg/ml | $322.00 | |||
MKP-4 Antibody (D-3) FITC | sc-137010 FITC | 200 µg/ml | $336.00 | |||
MKP-4 Antibody (D-3) PE | sc-137010 PE | 200 µg/ml | $349.00 | |||
MKP-4 Antibody (D-3) Alexa Fluor® 488 | sc-137010 AF488 | 200 µg/ml | $364.00 | |||
MKP-4 Antibody (D-3) Alexa Fluor® 546 | sc-137010 AF546 | 200 µg/ml | $364.00 | |||
MKP-4 Antibody (D-3) Alexa Fluor® 594 | sc-137010 AF594 | 200 µg/ml | $364.00 | |||
MKP-4 Antibody (D-3) Alexa Fluor® 647 | sc-137010 AF647 | 200 µg/ml | $364.00 | |||
MKP-4 Antibody (D-3) Alexa Fluor® 680 | sc-137010 AF680 | 200 µg/ml | $364.00 | |||
MKP-4 Antibody (D-3) Alexa Fluor® 790 | sc-137010 AF790 | 200 µg/ml | $364.00 |