MKP-3 Antibody (G-4): sc-137246

MKP-3 Antibody (G-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects MKP-3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-MKP-3 antibody (G-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MKP-3, a member of the dual specificity phosphatase family, plays a crucial role in regulating mitogen-activated protein (MAP) kinase signaling pathways, which are essential for cellular responses to growth factors and stress signals. By specifically dephosphorylating critical phosphothreonine and phosphotyrosine residues within MAP kinases, MKP-3 helps to modulate various physiological processes, including cell proliferation, differentiation, and apoptosis. MKP-3 expression is tightly regulated and can be induced by various stimuli, highlighting MKP-3′s importance in maintaining cellular homeostasis and preventing aberrant signaling that could lead to diseases such as cancer. Human MKP-3 is encoded by a gene located on chromosome 12q22-q23 and produces a 381 amino acid protein that is predominantly expressed in the heart and pancreas, underscoring MKP-3′s potential significance in these tissues.

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MKP-3 Antibody (G-4) References:

1. Intramolecular dephosphorylation of ERK by MKP3.  |  Kim, Y., et al. 2003. Biochemistry. 42: 15197-207. PMID: 14690430
2. ΔNp63α regulates Erk signaling via MKP3 to inhibit cancer metastasis.  |  Bergholz, J., et al. 2014. Oncogene. 33: 212-24. PMID: 23246965
3. MAP kinase phosphatase-3 (MKP-3) is transcriptionally and post-translationally up-regulated by hCG and modulates cAMP-induced p21 expression in MA-10 Leydig cells.  |  Mori Sequeiros García, M., et al. 2013. Mol Cell Endocrinol. 371: 174-81. PMID: 23261984
4. Mitogen-activated protein kinase (MAPK) phosphatase-3 (MKP-3) displays a p-JNK-MAPK substrate preference in astrocytes in vitro.  |  Ndong, C., et al. 2014. Neurosci Lett. 575: 13-8. PMID: 24861519
5. FOXO1-dependent up-regulation of MAP kinase phosphatase 3 (MKP-3) mediates glucocorticoid-induced hepatic lipid accumulation in mice.  |  Feng, B., et al. 2014. Mol Cell Endocrinol. 393: 46-55. PMID: 24946098
6. c-Myb negatively regulates Ras signaling through induction of dual phosphatase MKP-3 in NIH3T3 cells.  |  Park, YJ., et al. 2014. Biochem Biophys Res Commun. 450: 1032-7. PMID: 24978307
7. Mitogen-activated protein kinase phosphatase-3 (MKP-3) in the surgical wound is necessary for the resolution of postoperative pain in mice.  |  Skopelja-Gardner, S., et al. 2017. J Pain Res. 10: 763-774. PMID: 28405172
8. Hinokitiol suppresses growth of B16 melanoma by activating ERK/MKP3/proteosome pathway to downregulate survivin expression.  |  Wei, KC., et al. 2019. Toxicol Appl Pharmacol. 366: 35-45. PMID: 30684529
9. TRIM59, amplified in ovarian cancer, promotes tumorigenesis through the MKP3/ERK pathway.  |  Tong, X., et al. 2020. J Cell Physiol. 235: 8236-8245. PMID: 31951023
10. Catalytic activation of the phosphatase MKP-3 by ERK2 mitogen-activated protein kinase.  |  Camps, M., et al. 1998. Science. 280: 1262-5. PMID: 9596579

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MKP-3 Antibody (G-4) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-MKP-3 Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MKP-3 Antibody (G-4) Specifications

Alternate Product Name: MKP-3

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-130

Epitope Target: MKP-3

Epitope Species: human

Product Reactivity/Detection: MKP-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for MKP-3 Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred MKP-3 antibody is MKP-3 Antibody (F-12): sc-377070.