MITF Antibody (21D1418): sc-52938

MITF Antibody (21D1418) is a mouse monoclonal IgG1 kappa light chain antibody that detects MITF in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP). Anti-MITF antibody (21D1418) is available in a non-conjugated form, allowing for versatile applications in research. The microphthalmia-associated transcription factor (MITF) plays a crucial role in the development and differentiation of melanocytes, which are responsible for pigment production in the skin, hair, and eyes. MITF (21D1418) monoclonal antibody recognizes a protein essential for pigmentation and regulation of various genes involved in melanocyte function, including tyrosinase, TRP1, and TRP2, which are vital for melanin synthesis. The presence of basic helix-loop-helix (HLH) and leucine zipper (LZ) domains in MITF allows for homodimer formation and direct DNA binding, activating gene expression through E-box elements. MITF activity is modulated by post-translational modifications, such as phosphorylation on Ser73 via the MAPK pathway, which enhances interaction with co-activators from the p300/CBP family, thereby increasing transcriptional activity. MITF-deficient mice exhibit a complete absence of melanocytes, highlighting MITF′s indispensable role in melanocyte biology and pigmentation.

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MITF Antibody (21D1418) References:

1. Microphthalmia transcription factor. A sensitive and specific melanocyte marker for MelanomaDiagnosis.  |  King, R., et al. 1999. Am J Pathol. 155: 731-8. PMID: 10487831
2. MITF mediates cAMP-induced protein kinase C-beta expression in human melanocytes.  |  Park, HY., et al. 2006. Biochem J. 395: 571-8. PMID: 16411896
3. Inhibition of MITF transcriptional activity independent of targeting p300/CBP coactivators.  |  Vachtenheim, J., et al. 2007. Pigment Cell Res. 20: 41-51. PMID: 17250547
4. TFEB has DNA-binding and oligomerization properties of a unique helix-loop-helix/leucine-zipper family.  |  Fisher, DE., et al. 1991. Genes Dev. 5: 2342-52. PMID: 1748288
5. Sequence-specific DNA binding by Myc proteins.  |  Kerkhoff, E., et al. 1991. Proc Natl Acad Sci U S A. 88: 4323-7. PMID: 1827916
6. TFE3: a helix-loop-helix protein that activates transcription through the immunoglobulin enhancer muE3 motif.  |  Beckmann, H., et al. 1990. Genes Dev. 4: 167-79. PMID: 2338243
7. NNT mediates redox-dependent pigmentation via a UVB- and MITF-independent mechanism.  |  Allouche, J., et al. 2021. Cell. 184: 4268-4283.e20. PMID: 34233163
8. A central role for regulated protein stability in the control of TFE3 and MITF by nutrients.  |  Nardone, C., et al. 2023. Mol Cell. 83: 57-73.e9. PMID: 36608670
9. Acetylation reprograms MITF target selectivity and residence time.  |  Louphrasitthiphol, P., et al. 2023. Nat Commun. 14: 6051. PMID: 37770430
10. The basic helix-loop-helix-zipper domain of TFE3 mediates enhancer-promoter interaction.  |  Artandi, SE., et al. 1994. Mol Cell Biol. 14: 7704-16. PMID: 7969114
11. Molecular cloning of cDNA encoding a human TFEC isoform, a newly identified transcriptional regulator.  |  Yasumoto, K. and Shibahara, S. 1997. Biochim Biophys Acta. 1353: 23-31. PMID: 9256061
12. The bHLH-Zip transcription factor Tfeb is essential for placental vascularization.  |  Steingrímsson, E., et al. 1998. Development. 125: 4607-16. PMID: 9806910

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MITF Antibody (21D1418) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-MITF Antibody (21D1418) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MITF Antibody (21D1418) Specifications

Alternate Product Name: MITF

Product Clone Name: 21D1418

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 408-419

Epitope Target: MITF

Epitope Species: human

Product Reactivity/Detection: MITF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, bovine and canine

Additional Non-Mammalian Reactivity: Arabadopsis, Xenopus

Applications: WB, IP

Vialing Information: Each vial contains 50 µg IgG₁ kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for MITF Antibody (21D1418): N/A