MIST1 Antibody (A-6): sc-166181
- MIST1 Antibody (A-6) is a mouse monoclonal IgG2b κ MIST1 antibody provided at 200 µg/ml
- raised against amino acids 63-102 mapping within an internal region of MIST1 of human origin
- MIST1 Antibody (A-6) is recommended for detection of MIST1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-MIST1 Antibody (A-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for MIST1 Antibody (A-6) for WB and IHC(P) applications. This reagent is now offered in a bundle with MIST1 Antibody (A-6) (see ordering information below).
QUICK LINKS
SEE ALSO...
MIST1 Antibody (A-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects MIST1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-MIST1 antibody (A-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MIST1, also known as muscle, intestine, and stomach expression 1 or BHLHB8, is a 189 amino acid nuclear protein predominantly expressed in liver, brain, skeletal muscle, and spleen. MIST1 features a basic helix-loop-helix (bHLH) domain, which is crucial for functioning as a transcription factor. MIST1 binds to E-box motifs in promoter or enhancer regions of developmentally regulated genes, functioning as either a transcriptional activator or repressor. MIST1 interacts with E-proteins such as E12 and E47, and plays a significant role in regulating transcriptional activity of MyoD, a key protein in muscle cell development. MIST1 ensures myoblast populations remain undifferentiated by acting as a repressor of MyoD activity, which is vital for muscle development. MIST1 is essential for regulating mammary gland development, highlighting its importance in both muscle and epithelial cell differentiation processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MIST1 Antibody (A-6) References:
- Mist1 regulates pancreatic acinar cell proliferation through p21 CIP1/WAF1. | Jia, D., et al. 2008. Gastroenterology. 135: 1687-97. PMID: 18762186
- Silencing Mist1 Gene Expression Is Essential for Recovery from Acute Pancreatitis. | Karki, A., et al. 2015. PLoS One. 10: e0145724. PMID: 26717480
- CXCR4-expressing Mist1+ progenitors in the gastric antrum contribute to gastric cancer development. | Sakitani, K., et al. 2017. Oncotarget. 8: 111012-111025. PMID: 29340033
- Mist1 Expression Is Required for Paneth Cell Maturation. | Dekaney, CM., et al. 2019. Cell Mol Gastroenterol Hepatol. 8: 549-560. PMID: 31330316
- Mist1+ gastric isthmus stem cells are regulated by Wnt5a and expand in response to injury and inflammation in mice. | Nienhüser, H., et al. 2021. Gut. 70: 654-665. PMID: 32709613
- Mist1 Inhibits Epithelial-Mesenchymal Transition in Gastric Adenocarcinoma via Downregulating the Wnt/β-catenin Pathway. | Xie, X., et al. 2021. J Cancer. 12: 4574-4584. PMID: 34149921
- Mist1 promoted inflammation in colitis model via K+-ATPase NLRP3 inflammasome by SNAI1. | Wang, T., et al. 2021. Pathol Res Pract. 224: 153511. PMID: 34214845
- DDX56 transcriptionally activates MIST1 to facilitate tumorigenesis of HCC through PTEN-AKT signaling. | Zhou, H., et al. 2022. Theranostics. 12: 6069-6087. PMID: 36168636
- The Xbp1-regulated transcription factor Mist1 restricts antibody secretion by restraining Blimp1 expression in plasma cells. | Wöhner, M., et al. 2022. Front Immunol. 13: 859598. PMID: 36618345
- MIST1 regulates endoplasmic reticulum stress-induced hepatic apoptosis as a candidate marker of fatty liver disease progression. | Hur, S., et al. 2024. Cell Death Dis. 15: 805. PMID: 39516480
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MIST1 Antibody (A-6) | sc-166181 | 200 µg/ml | $316.00 | |||
MIST1 Antibody (A-6): m-IgG2b BP-HRP Bundle | sc-548674 | 200 µg Ab; 10 µg BP | $354.00 | |||
MIST1 Antibody (A-6) AC | sc-166181 AC | 500 µg/ml, 25% agarose | $416.00 | |||
MIST1 Antibody (A-6) HRP | sc-166181 HRP | 200 µg/ml | $316.00 | |||
MIST1 Antibody (A-6) FITC | sc-166181 FITC | 200 µg/ml | $330.00 | |||
MIST1 Antibody (A-6) PE | sc-166181 PE | 200 µg/ml | $343.00 | |||
MIST1 Antibody (A-6) Alexa Fluor® 488 | sc-166181 AF488 | 200 µg/ml | $357.00 | |||
MIST1 Antibody (A-6) Alexa Fluor® 546 | sc-166181 AF546 | 200 µg/ml | $357.00 | |||
MIST1 Antibody (A-6) Alexa Fluor® 594 | sc-166181 AF594 | 200 µg/ml | $357.00 | |||
MIST1 Antibody (A-6) Alexa Fluor® 647 | sc-166181 AF647 | 200 µg/ml | $357.00 | |||
MIST1 Antibody (A-6) Alexa Fluor® 680 | sc-166181 AF680 | 200 µg/ml | $357.00 | |||
MIST1 Antibody (A-6) Alexa Fluor® 790 | sc-166181 AF790 | 200 µg/ml | $357.00 |