MIST1 Antibody (6E8): sc-80984
- MIST1 Antibody (6E8) is a mouse monoclonal IgG1 κ, cited in 11 publications, provided at 200 µg/ml
- raised against amino acids 175-197 corresponding to the C-terminus of MIST1 of mouse origin
- Anti-MIST1 Antibody (6E8) is recommended for detection of MIST1 of mouse and rat origin by WB, IP, IF and IHC(P)
- Anti-MIST1 Antibody (6E8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MIST1 Antibody (6E8) for WB and IHC(P) applications. These reagents are now offered in bundles with MIST1 Antibody (6E8) (see ordering information below).
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MIST1 Antibody (6E8) is a mouse monoclonal IgG1 kappa light chain antibody that detects MIST1 of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-MIST1 antibody (6E8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MIST1, also known as muscle, intestine, and stomach expression 1 or BHLHB8, is a 189 amino acid nuclear protein predominantly expressed in the liver, brain, skeletal muscle, and spleen. MIST1 features a basic helix-loop-helix (bHLH) domain, which is crucial for its role as a transcription factor. The bHLH domain allows MIST1 to bind to E-box motifs in the promoter or enhancer regions of various developmentally regulated genes, functioning as either a transcriptional activator or repressor. MIST1 interacts with E-proteins such as E12 and E47, and plays a significant role in regulating the transcriptional activity of MyoD, a key protein in muscle cell development. By acting as a repressor of MyoD activity, MIST1 ensures that myoblast populations remain undifferentiated, which is vital for muscle development. MIST1 is essential for the regulation of mammary gland development, highlighting its importance in both muscle and epithelial cell differentiation processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MIST1 Antibody (6E8) References:
- The bHLH transcription factor Mist1 is required to maintain exocrine pancreas cell organization and acinar cell identity. | Pin, CL., et al. 2001. J Cell Biol. 155: 519-30. PMID: 11696558
- Sgn1, a basic helix-loop-helix transcription factor delineates the salivary gland duct cell lineage in mice. | Yoshida, S., et al. 2001. Dev Biol. 240: 517-30. PMID: 11784080
- Exhaustive identification of human class II basic helix-loop-helix proteins by virtual library screening. | McLellan, AS., et al. 2002. Mech Dev. 119 Suppl 1: S285-91. PMID: 14516699
- Identification of a basic helix-loop-helix transcription factor expressed in mammary gland alveolar cells and required for maintenance of the differentiated state. | Zhao, Y., et al. 2006. Mol Endocrinol. 20: 2187-98. PMID: 16645041
- Cloning, expression, and functional characterization of zebrafish Mist1. | Guo, X., et al. 2007. Biochem Biophys Res Commun. 359: 20-6. PMID: 17531198
- The bHLH domain of Mistl is sufficient to activate gene transcription. | Tran, T., et al. 2007. Gene Expr. 13: 241-53. PMID: 17605298
- A molecular signature of gastric metaplasia arising in response to acute parietal cell loss. | Nozaki, K., et al. 2008. Gastroenterology. 134: 511-22. PMID: 18242217
- Mist1: a novel basic helix-loop-helix transcription factor exhibits a developmentally regulated expression pattern. | Lemercier, C., et al. 1997. Dev Biol. 182: 101-13. PMID: 9073453
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MIST1 Antibody (6E8) | sc-80984 | 200 µg/ml | $322.00 | |||
MIST1 Antibody (6E8): m-IgG Fc BP-HRP Bundle | sc-528700 | 200 µg Ab; 10 µg BP | $361.00 | |||
MIST1 Antibody (6E8): m-IgGκ BP-HRP Bundle | sc-521163 | 200 µg Ab, 40 µg BP | $361.00 | |||
MIST1 Antibody (6E8): m-IgG1 BP-HRP Bundle | sc-543071 | 200 µg Ab; 20 µg BP | $361.00 | |||
MIST1 Antibody (6E8) AC | sc-80984 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MIST1 Antibody (6E8) HRP | sc-80984 HRP | 200 µg/ml | $322.00 | |||
MIST1 Antibody (6E8) FITC | sc-80984 FITC | 200 µg/ml | $336.00 | |||
MIST1 Antibody (6E8) PE | sc-80984 PE | 200 µg/ml | $349.00 | |||
MIST1 Antibody (6E8) Alexa Fluor® 488 | sc-80984 AF488 | 200 µg/ml | $364.00 | |||
MIST1 Antibody (6E8) Alexa Fluor® 546 | sc-80984 AF546 | 200 µg/ml | $364.00 | |||
MIST1 Antibody (6E8) Alexa Fluor® 594 | sc-80984 AF594 | 200 µg/ml | $364.00 | |||
MIST1 Antibody (6E8) Alexa Fluor® 647 | sc-80984 AF647 | 200 µg/ml | $364.00 | |||
MIST1 Antibody (6E8) Alexa Fluor® 680 | sc-80984 AF680 | 200 µg/ml | $364.00 | |||
MIST1 Antibody (6E8) Alexa Fluor® 790 | sc-80984 AF790 | 200 µg/ml | $364.00 |