Date published: 2026-4-27

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MIST1 Antibody (1H1): sc-80983

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Datasheets
  • MIST1 Antibody (1H1) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
  • raised against amino acids 1-20 corresponding to the N-terminus of MIST1 of mouse origin
  • recommended for detection of MIST1 of mouse and rat origin by WB, IP, IF and IHC(P)
  • See MIST1 (6E8): sc-80984 for MIST1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for MIST1 Antibody (1H1) for WB and IHC(P) applications. These reagents are now offered in bundles with MIST1 Antibody (1H1) (see ordering information below).
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    MIST1 Antibody (1H1) is a mouse monoclonal IgG1 antibody that detects MIST1 in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. MIST1, also known as BHLHB8, is a 189 amino acid nuclear protein predominantly expressed in the liver, brain, skeletal muscle, and spleen, where MIST1 plays a crucial role in regulating gene expression during development. MIST1 contains a basic helix-loop-helix (bHLH) domain, which allows MIST1 to bind to E-box motifs in the promoter or enhancer regions of various developmentally regulated genes, functioning as either a transcriptional activator or repressor. Notably, MIST1 acts as a repressor of MyoD, a key protein involved in muscle cell differentiation, thereby ensuring that myoblast populations remain undifferentiated and available for muscle development. Additionally, MIST1 is essential for the proper regulation of mammary gland development, highlighting MIST1′s importance in both muscle and epithelial cell biology. Anti-MIST1 antibody (1H1) is an invaluable tool for researchers studying these critical biological processes and the underlying mechanisms of muscle and mammary gland development.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    MIST1 Antibody (1H1)

    sc-80983
    200 µg/ml
    $322.00

    MIST1 Antibody (1H1): m-IgG Fc BP-HRP Bundle

    sc-539578
    200 µg Ab; 10 µg BP
    $361.00

    MIST1 Antibody (1H1): m-IgG1 BP-HRP Bundle

    sc-541570
    200 µg Ab; 20 µg BP
    $361.00

    I am using MIST1 (1H1): sc-80983 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

    Asked by: Professor Griffin
    We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-02-24
    • y_2026, m_4, d_25, h_11CST
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    Rated 5 out of 5 by from Great for IF on frozen sectionsWorked well for immunofluorescence on frozen sections at a dilution of 1:250.
    Date published: 2017-07-20
    Rated 4 out of 5 by from Produced great immunoperoxidase nuclear stainingProduced great immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded mouse pancreas tissue. -SCBT QC
    Date published: 2015-05-09
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    MIST1 Antibody (1H1) is rated 4.3 out of 5 by 3.
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