Misato Antibody (E-10): sc-390638

Misato Antibody (E-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects Misato protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Misato (E-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Misato protein plays a crucial role in cellular processes, particularly in regulating microtubule dynamics, which is essential for proper cell division and intracellular transport. Misato′s unique structure, which incorporates motifs found in alpha-, beta-, and gamma-tubulins, as well as elements related to myosin heavy chain proteins, highlights Misato′s importance in maintaining cellular integrity and function. Disruptions in misato gene can lead to severe developmental consequences, such as irregular chromosomal segregation during cell division, resulting in reduced brain size and larval mortality in Drosophila melanogaster, underscoring Misato′s significance in developmental biology and potential implications in understanding similar processes in higher eukaryotes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Misato Antibody (E-10) References:

1. The genome sequence of Drosophila melanogaster.  |  Adams, MD., et al. 2000. Science. 287: 2185-95. PMID: 10731132
2. An essential cell division gene of Drosophila, absent from Saccharomyces, encodes an unusual protein with tubulin-like and myosin-like peptide motifs.  |  Miklos, GL., et al. 1997. Proc Natl Acad Sci U S A. 94: 5189-94. PMID: 9144213
3. Structure of the alpha beta tubulin dimer by electron crystallography.  |  Nogales, E., et al. 1998. Nature. 391: 199-203. PMID: 9428769

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Misato Antibody (E-10) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Misato Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Misato Antibody (E-10) Specifications

Alternate Product Name: Misato

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-76

Epitope Target: Misato

Epitope Species: human

Product Reactivity/Detection: Misato

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Misato Antibody (E-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin