Date published: 2026-3-3

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MIPP Antibody (A-8): sc-514214

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Datasheets
  • MIPP Antibody (A-8) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
  • raised against amino acids 1-95 mapping at the N-terminus of MIPP of human origin
  • MIPP Antibody (A-8) is recommended for detection of MIPP of human origin by WB, IP, IF and ELISA
  • Anti-MIPP Antibody (A-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MIPP Antibody (A-8) for WB applications. These reagents are now offered in bundles with MIPP Antibody (A-8) (see ordering information below).
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MIPP Antibody (A-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects MIPP in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MIPP monoclonal antibody (A-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MIPP, or multiple inositol polyphosphate phosphatase, plays a crucial role in cellular signaling by hydrolyzing inositol polyphosphates, which are important second messengers in various biological processes. MIPP is primarily located in the cytoplasm and endoplasmic reticulum, where MIPP is involved in regulating inositol phosphate levels, thus influencing pathways related to cell growth, differentiation, and apoptosis. MIPP′s presence in these cellular compartments underscores MIPP′s significance in maintaining cellular homeostasis and responding to extracellular signals. MIPP is mapped to a region of chromosome 10 that is frequently mutated in human cancers, highlighting MIPP′s potential role in tumorigenesis. Originally isolated from rat liver, MIPP is also highly expressed in rat kidney, and MIPP′s evolutionary conservation across species, including mouse, chick, and fruit fly, suggests a fundamental role in cellular function throughout higher eukaryotes.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

MIPP Antibody (A-8)

sc-514214
200 µg/ml
$322.00

MIPP Antibody (A-8): m-IgG Fc BP-HRP Bundle

sc-531067
200 µg Ab; 10 µg BP
$361.00

MIPP Antibody (A-8): m-IgGκ BP-HRP Bundle

sc-524650
200 µg Ab, 40 µg BP
$361.00

MIPP Antibody (A-8): m-IgG1 BP-HRP Bundle

sc-544382
200 µg Ab; 20 µg BP
$361.00

MIPP Antibody (A-8) AC

sc-514214 AC
500 µg/ml, 25% agarose
$424.00

MIPP Antibody (A-8) HRP

sc-514214 HRP
200 µg/ml
$322.00

MIPP Antibody (A-8) FITC

sc-514214 FITC
200 µg/ml
$336.00

MIPP Antibody (A-8) PE

sc-514214 PE
200 µg/ml
$349.00

MIPP Antibody (A-8) Alexa Fluor® 488

sc-514214 AF488
200 µg/ml
$364.00

MIPP Antibody (A-8) Alexa Fluor® 546

sc-514214 AF546
200 µg/ml
$364.00

MIPP Antibody (A-8) Alexa Fluor® 594

sc-514214 AF594
200 µg/ml
$364.00

MIPP Antibody (A-8) Alexa Fluor® 647

sc-514214 AF647
200 µg/ml
$364.00

MIPP Antibody (A-8) Alexa Fluor® 680

sc-514214 AF680
200 µg/ml
$364.00

MIPP Antibody (A-8) Alexa Fluor® 790

sc-514214 AF790
200 µg/ml
$364.00

What is the recommended starting dilution for Western blot for MIPP (A-8): sc-514214 monoclonal antibody?

Asked by: Cweed
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-514214.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Works wellThis antibody works really well for MCF7, a human breast cancer cell line.
Date published: 2017-11-16
Rated 5 out of 5 by from Good antibodyUsed for WB, and it worked well. Also tried MIPP Antibody (A-4): sc-374456. For human cell lysates (I used MCF-7), (A-8) is better than (A-4).
Date published: 2017-11-02
Rated 5 out of 5 by from niceIn glioblastoma cells, the specific band at 50 kD was detected. There are no ns bands. 20 ug/ lane, 1/1000 dil in 2% BSA TBST, o/n reaction.
Date published: 2017-07-05
Rated 5 out of 5 by from Great Western blot data of MIPP expressionGreat Western blot data of MIPP expression in Hep G2, MIA PaCa-2, HeLa and K-562 whole cell lysates. -SCBT QC
Date published: 2013-10-15
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MIPP Antibody (A-8) is rated 5.0 out of 5 by 4.
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