MIPP Antibody (A-4): sc-374456
- MIPP Antibody (A-4) is a mouse monoclonal IgG1 κ MIPP antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 21-59 near the N-terminus of MIPP of mouse origin
- MIPP Antibody (A-4) is recommended for detection of MIPP of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA
- Anti-MIPP Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for MIPP Antibody (A-4) for WB applications. These reagents are now offered in bundles with MIPP Antibody (A-4) (see ordering information below).
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MIPP Antibody (A-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects MIPP protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MIPP (A-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MIPP protein, or multiple inositol polyphosphate phosphatase, plays a crucial role in cellular signaling by hydrolyzing inositol polyphosphates, which are important second messengers in various biological processes. MIPP′s enzymatic activity is vital for regulating cellular functions such as cell growth, differentiation, and apoptosis. MIPP′s unique ability to hydrolyze inositol pentakisphosphate and inositol hexakisphosphate highlights MIPP′s significance in the inositol phosphate signaling pathway, which is implicated in numerous physiological and pathological conditions, including cancer. Evolutionary conservation of MIPP across species, including mouse (MMU)Minpp1, human (HSA)MINPP1, chick, plant, and fruit fly, underscores MIPP′s essential role in higher eukaryotes. MIPP is located on chromosome 10, a region frequently associated with mutations in human cancers, and MIPP′s C-terminal domain contains a signal that retains soluble proteins within the endoplasmic reticulum, further emphasizing MIPP′s importance in cellular homeostasis. Originally isolated from rat liver, MIPP is also highly expressed in rat kidney, indicating MIPP′s diverse functional roles in different tissues.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MIPP Antibody (A-4) References:
- Multiple inositol polyphosphate phosphatase: evolution as a distinct group within the histidine phosphatase family and chromosomal localization of the human and mouse genes to chromosomes 10q23 and 19. | Chi, H., et al. 1999. Genomics. 56: 324-36. PMID: 10087200
- Hepatic Ins(1,3,4,5)P4 3-phosphatase is compartmentalized inside endoplasmic reticulum. | Ali, N., et al. 1993. J Biol Chem. 268: 6161-7. PMID: 8384201
- Molecular cloning and expression of a rat hepatic multiple inositol polyphosphate phosphatase. | Craxton, A., et al. 1997. Biochem J. 328 (Pt 1): 75-81. PMID: 9359836
- HiPER1, a phosphatase of the endoplasmic reticulum with a role in chondrocyte maturation. | Romano, PR., et al. 1998. J Cell Sci. 111 (Pt 6): 803-13. PMID: 9472008
- The human and rat forms of multiple inositol polyphosphate phosphatase: functional homology with a histidine acid phosphatase up-regulated during endochondral ossification. | Caffrey, JJ., et al. 1999. FEBS Lett. 442: 99-104. PMID: 9923613
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MIPP Antibody (A-4) | sc-374456 | 200 µg/ml | $316.00 | |||
MIPP Antibody (A-4): m-IgG Fc BP-HRP Bundle | sc-527467 | 200 µg Ab; 10 µg BP | $354.00 | |||
MIPP Antibody (A-4): m-IgG1 BP-HRP Bundle | sc-532840 | 200 µg Ab; 20 µg BP | $354.00 | |||
MIPP Antibody (A-4) AC | sc-374456 AC | 500 µg/ml, 25% agarose | $416.00 | |||
MIPP Antibody (A-4) HRP | sc-374456 HRP | 200 µg/ml | $316.00 | |||
MIPP Antibody (A-4) FITC | sc-374456 FITC | 200 µg/ml | $330.00 | |||
MIPP Antibody (A-4) PE | sc-374456 PE | 200 µg/ml | $343.00 | |||
MIPP Antibody (A-4) Alexa Fluor® 488 | sc-374456 AF488 | 200 µg/ml | $357.00 | |||
MIPP Antibody (A-4) Alexa Fluor® 546 | sc-374456 AF546 | 200 µg/ml | $357.00 | |||
MIPP Antibody (A-4) Alexa Fluor® 594 | sc-374456 AF594 | 200 µg/ml | $357.00 | |||
MIPP Antibody (A-4) Alexa Fluor® 647 | sc-374456 AF647 | 200 µg/ml | $357.00 | |||
MIPP Antibody (A-4) Alexa Fluor® 680 | sc-374456 AF680 | 200 µg/ml | $357.00 | |||
MIPP Antibody (A-4) Alexa Fluor® 790 | sc-374456 AF790 | 200 µg/ml | $357.00 | |||
MIPP (A-4) Neutralizing Peptide | sc-374456 P | 100 µg/0.5 ml | $68.00 |