MIB1 Antibody (B-9): sc-393811

MIB1 Antibody (B-9) is a mouse monoclonal IgG1 antibody that detects MIB1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MIB1, also known as Mind bomb homolog 1 and DAPK-interacting protein 1, is a crucial E3 ubiquitin ligase composed of 1006 amino acids that plays a significant role in the regulation of Notch signaling by activating the Notch ligand, Delta. By binding to the intracellular domain of Delta, MIB1 facilitates its ubiquitination, leading to the endocytosis and degradation of the Delta receptor. This process, while seemingly counterintuitive, ultimately enhances Notch signaling, which is vital for proper cell fate decisions during development. MIB1′s interaction with DAPK, a protein involved in apoptosis regulation, further underscores MIB1′s importance; the ubiquitination of DAPK by MIB1 inhibits caspase-dependent apoptosis, suggesting that elevated levels of MIB1 could contribute to tumor growth. Notably, MIB1 is predominantly expressed in the central nervous system and ovary, with both MIB1 and DAPK being overexpressed in epileptic brain tissue, indicating their potential collaborative role in regulating neuronal death in epilepsy. anti-MIB1 antibody (B-9) is an essential tool for researchers investigating the intricate mechanisms of cell signaling and apoptosis in various biological contexts.

The preferred MIB1 antibody is MIB1 Antibody (D-6): sc-393551.