MIB1 Antibody (D-6): sc-393551
- MIB1 Antibody (D-6) is a mouse monoclonal IgG2b κ MIB1 antibody, cited in 6 publications, provided at 200 µg/ml
- raised against amino acids 356-566 mapping within an internal region of MIB1 of human origin
- MIB1 Antibody (D-6) is recommended for detection of MIB1 of mouse, rat, human and origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-MIB1 Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MIB1 Antibody (D-6) for WB applications. These reagents are now offered in bundles with MIB1 Antibody (D-6) (see ordering information below).
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MIB1 Antibody (D-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects MIB1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MIB1 (D-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MIB1, also known as E3 ubiquitin-protein ligase MIB1 or Mind bomb homolog 1, is a crucial 1006 amino acid E3 ubiquitin ligase that plays a significant role in activating the Notch signaling pathway by ubiquitylating the Notch ligand, Delta. This process involves MIB1 binding to the intracellular domain of Delta, facilitating endocytosis and subsequent degradation of the Delta receptor, ultimately leading to increased Notch receptor activity. This regulatory mechanism is vital for proper cell fate decisions during development, influencing various cellular processes, including differentiation and apoptosis. MIB1 is predominantly expressed in the central nervous system and ovary, and MIB1′s interaction with DAPK, a protein involved in apoptosis regulation, suggests a complex interplay that may contribute to tumor growth when MIB1 is overexpressed. Notably, MIB1 and DAPK are found to be overexpressed in epileptic brain tissue, indicating their potential cooperative role in regulating neuronal death in epilepsy.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MIB1 Antibody (D-6) References:
- A death-associated protein kinase (DAPK)-interacting protein, DIP-1, is an E3 ubiquitin ligase that promotes tumor necrosis factor-induced apoptosis and regulates the cellular levels of DAPK. | Jin, Y., et al. 2002. J Biol Chem. 277: 46980-6. PMID: 12351649
- Mind bomb is a ubiquitin ligase that is essential for efficient activation of Notch signaling by Delta. | Itoh, M., et al. 2003. Dev Cell. 4: 67-82. PMID: 12530964
- Notch signaling: endocytosis makes delta signal better. | Le Borgne, R. and Schweisguth, F. 2003. Curr Biol. 13: R273-5. PMID: 12676105
- Death-associated protein kinase expression in human temporal lobe epilepsy. | Henshall, DC., et al. 2004. Ann Neurol. 55: 485-94. PMID: 15048887
- Neuronal morphogenesis is regulated by the interplay between cyclin-dependent kinase 5 and the ubiquitin ligase mind bomb 1. | Choe, EA., et al. 2007. J Neurosci. 27: 9503-12. PMID: 17728463
- Mind bomb 1-expressing intermediate progenitors generate notch signaling to maintain radial glial cells. | Yoon, KJ., et al. 2008. Neuron. 58: 519-31. PMID: 18498734
- Mind bomb-1 is essential for intraembryonic hematopoiesis in the aortic endothelium and the subaortic patches. | Yoon, MJ., et al. 2008. Mol Cell Biol. 28: 4794-804. PMID: 18505817
- Mind bomb 1 in the lymphopoietic niches is essential for T and marginal zone B cell development. | Song, R., et al. 2008. J Exp Med. 205: 2525-36. PMID: 18824586
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MIB1 Antibody (D-6) | sc-393551 | 200 µg/ml | $322.00 | |||
MIB1 Antibody (D-6): m-IgGκ BP-HRP Bundle | sc-523991 | 200 µg Ab, 40 µg BP | $361.00 | |||
MIB1 Antibody (D-6): m-IgG2b BP-HRP Bundle | sc-549148 | 200 µg Ab; 10 µg BP | $361.00 | |||
MIB1 Antibody (D-6) AC | sc-393551 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MIB1 Antibody (D-6) HRP | sc-393551 HRP | 200 µg/ml | $322.00 | |||
MIB1 Antibody (D-6) FITC | sc-393551 FITC | 200 µg/ml | $336.00 | |||
MIB1 Antibody (D-6) PE | sc-393551 PE | 200 µg/ml | $349.00 | |||
MIB1 Antibody (D-6) Alexa Fluor® 488 | sc-393551 AF488 | 200 µg/ml | $364.00 | |||
MIB1 Antibody (D-6) Alexa Fluor® 546 | sc-393551 AF546 | 200 µg/ml | $364.00 | |||
MIB1 Antibody (D-6) Alexa Fluor® 594 | sc-393551 AF594 | 200 µg/ml | $364.00 | |||
MIB1 Antibody (D-6) Alexa Fluor® 647 | sc-393551 AF647 | 200 µg/ml | $364.00 | |||
MIB1 Antibody (D-6) Alexa Fluor® 680 | sc-393551 AF680 | 200 µg/ml | $364.00 | |||
MIB1 Antibody (D-6) Alexa Fluor® 790 | sc-393551 AF790 | 200 µg/ml | $364.00 |