MIA3 Antibody (C-5): sc-393916

MIA3 Antibody (C-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects MIA3 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MIA3 Antibody (C-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MIA3, also known as melanoma inhibitory activity family, member 3, ARNT, or TANGO, is a 1,907 amino acid single-pass type I membrane protein primarily localized to the endoplasmic reticulum (ER). Such localization is crucial as MIA3 plays a significant role in collagen type VII (COL7A1) secretion and cargo loading at ER exit sites, essential processes for maintaining cellular homeostasis and proper extracellular matrix formation. MIA3 is expressed in various tissues, excluding bone marrow and peripheral blood mononuclear cells, and regulates protein release from the ER, highlighting MIA3′s importance in protein trafficking and secretion. MIA3 gene is located on human chromosome 1, which spans 260 million base pairs and contains over 3,000 genes, including many associated with various diseases such as familial adenomatous polyposis and multiple myeloma. Aberrations in chromosome 1 are frequently observed in several cancers, underscoring MIA3′s potential significance in oncogenic processes.

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MIA3 Antibody (C-5) References:

1. Characterization and expression pattern of the novel MIA homolog TANGO.  |  Bosserhoff, AK., et al. 2004. Gene Expr Patterns. 4: 473-9. PMID: 15183315
2. TANGO is a tumor suppressor of malignant melanoma.  |  Arndt, S. and Bosserhoff, AK. 2006. Int J Cancer. 119: 2812-20. PMID: 17044017
3. Interactions of TANGO and leukocyte integrin CD11c/CD18 regulate the migration of human monocytes.  |  Arndt, S., et al. 2007. J Leukoc Biol. 82: 1466-72. PMID: 17726152
4. Reduced expression of TANGO in colon and hepatocellular carcinomas.  |  Arndt, S. and Bosserhoff, AK. 2007. Oncol Rep. 18: 885-91. PMID: 17786351
5. TANGO1 facilitates cargo loading at endoplasmic reticulum exit sites.  |  Saito, K., et al. 2009. Cell. 136: 891-902. PMID: 19269366
6. MicroRNA-222 influences migration and invasion through MIA3 in colorectal cancer.  |  Gao, H., et al. 2017. Cancer Cell Int. 17: 78. PMID: 28855850
7. A general role for TANGO1, encoded by MIA3, in secretory pathway organization and function.  |  McCaughey, J., et al. 2021. J Cell Sci. 134: PMID: 34350936
8. MIA3 Splice Defect in Cane Corso Dogs with Dental-Skeletal-Retinal Anomaly (DSRA).  |  Christen, M., et al. 2021. Genes (Basel). 12: PMID: 34680893
9. Association study between polymorphisms in MIA3, SELE, SMAD3 and CETP genes and coronary artery disease in an Iranian population.  |  Rayat, S., et al. 2022. BMC Cardiovasc Disord. 22: 298. PMID: 35768776
10. A novel mutation in collagen transport protein, MIA3 gene, detected in a patient with clinical symptoms of Ehlers-Danlos hypermobile syndrome.  |  Junkiert-Czarnecka, A., et al. 2023. Adv Clin Exp Med. 32: 777-781. PMID: 36603143
11. MIA3 promotes the degradation of GSH (glutathione) by binding to CHAC1, thereby promoting the progression of hepatocellular carcinoma.  |  Wanbiao, Z., et al. 2024. Mol Cell Biochem. 479: 2769-2784. PMID: 37948019
12. Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain.  |  Nagase, T., et al. 1996. DNA Res. 3: 321-9, 341-54. PMID: 9039502

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MIA3 Antibody (C-5) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-MIA3 Antibody (C-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MIA3 Antibody (C-5) Specifications

Alternate Product Name: MIA3

Product Clone Name: C-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1391-1546

Epitope Target: MIA3

Epitope Species: human

Product Reactivity/Detection: MIA3

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for MIA3 Antibody (C-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin