MHC class II Antibody (Y-Ae) recommended for detection of MHC class II of mouse origin by WB, IP, IF, IHC(P) and FCM

MHC class II Antibody (Y-Ae): sc-32247

MHC class II Antibody (Y-Ae) is rated 5.0 out of 5 by 3.
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Datasheets

    • Anti-MHC class II Antibody (Y-Ae) is a mouse monoclonal IgG2b (lambda light chain) MHC class II antibody provided at 200 µg/ml
    • raised against lipopolysaccharide activated spleen cells of mouse origin
    • MHC class II Antibody (Y-Ae) is recommended for detection of splenic B cells expressing the I-Ab MHC class II molecules complexed to the 52-68 fragment of the α chain of I-E class II molecules (the Eα 52-68 peptide) of mouse origin by WB, IP, IF, IHC(P) and FCM
    • Anti-MHC class II Antibody (Y-Ae) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MHC class II (Y-Ae): sc-32247.
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How can I save this antibody for longer?

Asked by: mengting jin
Thank you for your inquiry. All of our products are warrantied for one year from the date purchased. If you have any further questions, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Service 11
Date published: 2018-01-09

Can this antibody be used with human samples?

Asked by: AbPolly
Thank you for your question. This MHC class II antibody is not recommended for use with human samples. For homology and publication information, and to discuss other available antibodies that are reactive with human, please contact our Technical Service Department.
Answered by: Technical Service
Date published: 2017-01-27
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Rated 5 out of 5 by from Produced publishable FCM data on mouse and human Strong antibody, highly recommended for a variety of applications, several references available in the primary literature for Flow on mouse control and tumor associated macrophages and human Dental follicle.
Date published: 2016-12-29
Rated 5 out of 5 by from Good specificity in WB in 1 Good specificity in WB in 1-11.15 whole cell lysate and mouse spleen tissue extract. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from Published IF data with mouse splenocytes Published IF data with mouse splenocytes - SCBT Publication Review
Date published: 2014-06-12
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