MHC class II Antibody (ER-TR3): sc-59318

MHC class II Antibody (ER-TR3) is a rat monoclonal IgG2b antibody that detects MHC class II in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). MHC class II (ER-TR3) antibody is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. Major histocompatibility complex (MHC) class II molecules play a crucial role in the immune response by presenting processed exogenous antigens to CD4+ T helper cells, which are essential for orchestrating adaptive immunity. These molecules are primarily located on the surface of antigen-presenting cells, including dendritic cells, macrophages, and B cells, where they facilitate the recognition of foreign peptides by T lymphocytes. The ability of MHC class II molecules to bind longer peptides, typically ranging from 13 to 18 amino acids, is vital for effective immune signaling, as this binding allows for a diverse array of antigens to be presented, thus enhancing the immune system′s ability to respond to various pathogens. The interaction between MHC class II and T cell receptors is a key event in the activation of helper T cells, which subsequently leads to the activation of B cells and cytotoxic T cells, underscoring MHC class II′s importance in maintaining immune homeostasis and response.

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MHC class II Antibody (ER-TR3) References:

1. MHC class II signaling in antigen-presenting cells.  |  Al-Daccak, R., et al. 2004. Curr Opin Immunol. 16: 108-13. PMID: 14734118
2. Chaperoning antigen presentation by MHC class II molecules and their role in oncogenesis.  |  Marsman, M., et al. 2005. Adv Cancer Res. 93: 129-58. PMID: 15797446
3. MHC class II regulation by epigenetic agents and microRNAs.  |  Tomasi, TB., et al. 2010. Immunol Res. 46: 45-58. PMID: 19771399
4. Routes to manipulate MHC class II antigen presentation.  |  van den Hoorn, T., et al. 2011. Curr Opin Immunol. 23: 88-95. PMID: 21112200
5. Angiotensin-converting enzyme affects the presentation of MHC class II antigens.  |  Zhao, T., et al. 2017. Lab Invest. 97: 764-771. PMID: 28394320
6. Murine cytomegalovirus degrades MHC class II to colonize the salivary glands.  |  Yunis, J., et al. 2018. PLoS Pathog. 14: e1006905. PMID: 29447285
7. Evolutionary Pressure against MHC Class II Binding Cancer Mutations.  |  Marty Pyke, R., et al. 2018. Cell. 175: 416-428.e13. PMID: 30245014
8. Footprints of antigen processing boost MHC class II natural ligand predictions.  |  Barra, C., et al. 2018. Genome Med. 10: 84. PMID: 30446001
9. Transplant outcome for MHC class II deficiency.  |  . 2020. Blood. 135: 975. PMID: 32191804
10. Ligation of MHC Class II Induces PKC-Dependent Clathrin-Mediated Endocytosis of MHC Class II.  |  Masaki, K., et al. 2020. Cells. 9: PMID: 32751549

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MHC class II Antibody (ER-TR3) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-MHC class II Antibody (ER-TR3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MHC class II Antibody (ER-TR3) Specifications

Alternate Product Name: Major Histocompatibility Complex Class 2

Product Clone Name: ER-TR3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: MHC Class II

Epitope Species: mouse

Product Reactivity/Detection: MHC class II

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for MHC class II Antibody (ER-TR3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred MHC class II antibody is MHC class II Antibody (IBL-5/22): sc-59322.