MHC class II Antibody (ER-TR3) recommended for detection of MHC class II of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA

MHC class II Antibody (ER-TR3): sc-59318

MHC class II Antibody (ER-TR3) is rated 4.0 out of 5 by 1.
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Datasheets

    • MHC class II Antibody (ER-TR3) is a rat monoclonal IgG2b provided at 200 µg/ml
    • raised against full length MHC Class II of mouse origin
    • MHC class II Antibody (ER-TR3) is recommended for detection of MHC class II of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA
    • MHC class II Antibody (ER-TR3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MHC class II (ER-TR3): sc-59318.
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Can sc-59318: MHC class II (ER-TR3) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, sc-59318: MHC class II (ER-TR3) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Publishable IHC (acetone) using human liver Publishable IHC (acetone) using human liver - SCBT Publication Review
Date published: 2013-03-08
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