MHC class I Antibody (ER-HR52): sc-59199

MHC class I Antibody (ER-HR52) is a rat monoclonal IgG2a antibody designed to detect Major Histocompatibility Complex class I (MHC I) molecules, also known as H-2 molecules in mice, on the surface of cells from various mouse haplotypes including H2-Db, H2-Dw16, H2d,p,q, and to a lesser extent, H2f,r,s,w17,w23,w27. Anti-MHC class I antibody (ER-HR52) targets MHC class I proteins located on the plasma membrane, which is essential for presenting endogenous peptides to cytotoxic T lymphocytes and plays a critical role in immune surveillance and response. MHC class I (ER-HR52) antibody is effective in multiple applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF), ensuring versatile use in diverse research settings. The epitope is raised against macrophage precursor cells of mouse origin, enhancing specificity and binding affinity for the designated MHC class I molecules. Available in both conjugated and unconjugated forms, MHC class I (ER-HR52) monoclonal antibody accommodates various detection methods and experimental designs. By specifically recognizing cell surface MHC class I proteins, anti-MHC class I antibody (ER-HR52) is invaluable for studies involving antigen presentation, immune cell interactions, and related immunological research.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MHC class I Antibody (ER-HR52) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-MHC class I Antibody (ER-HR52) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MHC class I Antibody (ER-HR52) Specifications

Alternate Product Name: Major Histocompatibility Complex class I

Product Clone Name: ER-HR52

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids:

Epitope Target: MHC class I

Epitope Species: mouse

Product Reactivity/Detection: MHC class I molecules on the surface of cells of the following haplotypes: H2-D^b, H2-D^w16, H2^d,p,q and, to a lesser extent, H2^{f,r,s,w17,w23,w27}

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for MHC class I Antibody (ER-HR52): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, PE: Phycoerythrin

The preferred MHC class I antibody is MHC class I Antibody (W6/32): sc-32235.