MHC class I Antibody (ER-HR52)recommended for detection of MHC class I molecules on the surface of cells of H2-Db, H2-Dw16, H2d,p,q and, to a lesser extent, H2f,r,s,w17,w23,w27 of mouse origin by WB, IP and IF
MHC class I Antibody (ER-HR52) is a rat monoclonal IgG2a provided at 200 µg/ml
raised against macrophage precursor cells of mouse origin
MHC class I Antibody (ER-HR52) is recommended for detection of MHC class I molecules on the surface of cells of the following haplotypes: H2-Db, H2-Dw16, H2d,p,q and, to a lesser extent, H2f,r,s,w17,w23,w27 of mouse origin by WB, IP and IF; non cross-reactive with MHC class I molecules of other haplotypes
MHC class I Antibody (ER-HR52) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MHC class I (ER-HR52): sc-59199.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Major histocompatibility complex (MHC) molecules, also designated human leukocyte antigen (HLA) molecules, are cell-surface receptors that bind foreign peptides and present them to T lymphocytes. MHC class I molecules consist of two polypeptide chains, an a or heavy chain, and β-2-Microglobulin, a non-covalently associated protein. Cytotoxic T lymphocytes bind antigenic peptides presented by MHC class I molecules. Antigens that bind to MHC class I molecules are typically 8-10 residues in length and are stabilized in a peptide binding groove. MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an a and b chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Can MHC class I (ER-HR52): sc-59199 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?
Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, MHC class I (ER-HR52): sc-59199 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Rated 5 out of
Worked great for murine dcsWorked great for murine dcs by flow cytometric detection!
Date published: 2019-12-04
Rated 5 out of
Produced publishable WB data in mouse and human.This MHC class I monoclonal antibody has several citations in the primary literature for the use in WB, IF and IHC on mouse and human cells.
H292,H3255,H441,HFL-1, MEF, Placental and intestinal tissue samples and mouse T-cells.
Date published: 2017-01-24
Rated 5 out of
published in 2011: IF(pfa) of mouse tpublished in 2011: IF(pfa) of mouse t-cells
Date published: 2015-11-06
Rated 5 out of
Robust signal in WB in CTLLRobust signal in WB in CTLL-2 whole cell lysate. -SCBT QC
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