MHC class I Antibody (ER-HR52) recommended for detection of MHC class I molecules on the surface of cells of H2-Db, H2-Dw16, H2d,p,q and, to a lesser extent, H2f,r,s,w17,w23,w27 of mouse origin by WB, IP and IF

MHC class I Antibody (ER-HR52): sc-59199

MHC class I Antibody (ER-HR52) is rated 5.0 out of 5 by 4.
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Datasheets

    • MHC class I Antibody (ER-HR52) is a rat monoclonal IgG2a provided at 200 µg/ml
    • raised against macrophage precursor cells of mouse origin
    • MHC class I Antibody (ER-HR52) is recommended for detection of MHC class I molecules on the surface of cells of the following haplotypes: H2-Db, H2-Dw16, H2d,p,q and, to a lesser extent, H2f,r,s,w17,w23,w27 of mouse origin by WB, IP and IF; non cross-reactive with MHC class I molecules of other haplotypes
    • MHC class I Antibody (ER-HR52) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MHC class I (ER-HR52): sc-59199.
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Can MHC class I (ER-HR52): sc-59199 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, MHC class I (ER-HR52): sc-59199 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Worked great for murine dcs Worked great for murine dcs by flow cytometric detection!
Date published: 2019-12-04
Rated 5 out of 5 by from Produced publishable WB data in mouse and human. This MHC class I monoclonal antibody has several citations in the primary literature for the use in WB, IF and IHC on mouse and human cells. H292,H3255,H441,HFL-1, MEF, Placental and intestinal tissue samples and mouse T-cells.
Date published: 2017-01-24
Rated 5 out of 5 by from published in 2011: IF(pfa) of mouse t published in 2011: IF(pfa) of mouse t-cells
Date published: 2015-11-06
Rated 5 out of 5 by from Robust signal in WB in CTLL Robust signal in WB in CTLL-2 whole cell lysate. -SCBT QC
Date published: 2014-06-27
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