MHC class I Antibody (BRA23/9) recommended for detection of MHC class I of human origin by WB, IP, IF and FCM

MHC class I Antibody (BRA23/9): sc-66205

MHC class I Antibody (BRA23/9) is rated 4.0 out of 5 by 1.
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Datasheets

    • MHC class I Antibody (BRA23/9) is a mouse monoclonal IgG2a provided at 200 µg/ml
    • raised against REH cell line of human origin
    • recommended for detection of MHC class I of human origin by WB, IP, IF and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See MHC class I (W6/32): sc-32235 for MHC class I antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can MHC class I (BRA23/9): sc-66205 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, MHC class I (BRA23/9): sc-66205 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Publishable WB and FCM using human MDA Publishable WB and FCM using human MDA-MB-231 and MCF-7 cell lines - SCBT Publication Review
Date published: 2013-09-19
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