MGMT Antibody (E-1): sc-166528

MGMT Antibody (E-1) is a mouse monoclonal IgG2a kappa light chain antibody specific for an epitope mapping between amino acids 9-24 at the N-terminus of human MGMT, showing reactivity with MGMT from mouse, rat, and human origins by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MGMT, also known as O6-methylguanine-DNA methyltransferase or O-6-alkylguanine DNA alkyltransferase (AGT), is a vital DNA repair enzyme that removes mutagenic and cytotoxic alkyl groups from the O6 position of guanine in DNA. Such alkylation lesions can lead to mispairing during DNA replication, resulting in mutations and potentially carcinogenesis. MGMT is predominantly located in the cell nucleus, where direct access to genomic DNA allows for efficient repair of DNA damage before cell division occurs. Nuclear localization ensures maintenance of genomic stability and prevents propagation of DNA errors to daughter cells. High levels of MGMT can confer resistance to alkylating chemotherapeutic agents, making MGMT expression significant in cancer research and therapy development. Anti-MGMT antibody (E-1) serves as a valuable tool for researchers investigating DNA repair mechanisms and MGMT expression in cancer treatment outcomes.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MGMT Antibody (E-1) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-MGMT Antibody (E-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MGMT Antibody (E-1) Specifications

Alternate Product Name: MGMT

Product Clone Name: E-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 9-24

Epitope Target: MGMT

Epitope Species: human

Product Reactivity/Detection: MGMT

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for MGMT Antibody (E-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred MGMT antibody is MGMT Antibody (MT 3.1): sc-56157.